Beta sheet tapes ribbons in tissue engineering
There is described a material comprising tapes, ribbons, fibrils or fibres characterized in that each of the ribbons, fibrils or fibres have an antiparallel arrangement of peptides in a β-sheet tape-like substructure.
1. A self assembling polypeptide (SAP) that forms ribbons, fibrils or fibres in a β-sheet tape-like substructure at physiological pH and physiological salt concentrations, and wherein the polypeptide is 11 residues in length and comprises P 11-15 (NNRFEWEFENN, SEQ ID NO: 7).
2. A method of forming a tape, comprising contacting the SAP of claim 1 with an ionic aqueous medium, thereby forming a tape.
3. A method of forming a gel, comprising contacting the SAP of claim 1 with an ionic aqueous medium, thereby forming a gel.
4. A composition, comprising the self-assembling polypeptide of claim 1 , and a physiologically acceptable salt concentration.
5. A dental product, comprising the self-assembling polypeptide of claim 1 .
6. The dental product of claim 5 , wherein the dental product is a dental repair or reconstruction product.
7. A tissue engineering scaffold, comprising the self-assembling polypeptide of claim 1 .
8. A hair care product, comprising the self-assembling polypeptide of claim 1 .
9. A composition, comprising: ribbons, fibrils or fibres, wherein each of the ribbons, fibrils or fibres has an antiparallel arrangement of peptides in a β-sheet tape-like substructure at physiological pH and physiological salt concentrations, wherein each peptide or pair of complementary peptides comprises a net =2 or a +2 charge, and wherein the peptide comprises P 11-15 (NNRFEWEFENN, SEQ ID NO: 7); and
a physiologically acceptable salt concentration.
10. The composition of claim 9 , wherein the composition comprises fibrils, and wherein the fibrils are comprised in a network of fibrils interconnected at fibre-like junctions.
11. The composition of claim 9 , wherein the composition comprises a dental product.
12. The composition of claim 11 , wherein the dental product is a dental repair or reconstruction product.
13. The composition of claim 9 , wherein the composition is in the form of a tissue engineering scaffold.
14. The composition according to claim 13 , comprising the scaffold seeded with cells.
15. The composition of claim 9 , wherein the composition comprises a skin treatment.
16. The composition of claim 9 , wherein the composition comprises a hair care product.
17. The composition of claim 9 , wherein the composition is part of a bioresponsive and biocompatible surface.
18. The composition of claim 9 , wherein the composition comprises continuous filament yarns, staple, floc, cord, or fabric.
19. The composition of claim 9 , further comprising a polymer.
20. The composition of claim 9 , wherein the peptide is at a concentration of 15 mg/ml to 35 mg/ml in the composition.
21. The composition of claim 9 , wherein the composition is at a physiological pH.
22. The composition of claim 9 , wherein the physiologically acceptable salt concentration is about 145 mM NaCl.
23. A method of forming a tape, comprising contacting the composition of claim 9 with an ionic aqueous medium, thereby forming a tape.
24. The composition of claim 9 , wherein the composition is at a physiological pH, and wherein the peptide is present at a concentration of greater than 15 mg/ml in the composition.
25. The composition of claim 21 , wherein the physiological pH is about 7.5.