Granted Patent
B2
US 9,115,163 · App. 12/734,229 · Granted Aug 25, 2015
DNA sequence with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators
Inventors:
Jingyue Ju (Englewood Cliffs, NJ); Dae H. Kim (Santa Clara, CA); Jia Guo (New York, NY); Qinglin Meng (Sunnyvale, CA); Zengmin Li (New York, NY); Huanyan Cao (New York, NY)
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Abstract
This invention provides a process for sequencing nucleic acids using 3′ modified deoxynucleotide analogues or 3′ modified deoxyinosine triphosphate analogues, and 3′ modified dideoxynucleotide analogues having a detectable marker attached to a base thereof.
Claims (4)
1. A compound having the structure:
wherein R′ is a cleavable chemical group; and wherein the base may have a detectable marker cleavably linked to it.
2. The compound of claim 1 , wherein R′ is a nitrobenzyl group, an allyl group or a methylazido group.
3. The compound of claim 1 , wherein the base has a detectable marker cleavably linked thereto.
Assignments (2)
CONFIRMATORY LICENSE
Recorded Jan 6, 2011
From: COLUMBIA UNIV NEW YORK MORNINGSIDE
To: NATIONAL INSTITUTES OF HEALTH (NIH), U.S. DEPT. OF HEALTH AND HUMAN SERVICES (DHHS), U.S. GOVERNMENT
Reel/Frame 025590/0816 →
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Sep 13, 2010
From: JU, JINGYUE; KIM, DAE H.; GUO, JIA; MENG, QINGLIN; LI, ZENGMIN; CAO, HUANYAN
To: TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK, THE
Reel/Frame 024976/0265 →
Continuity (3)
Provisional Application
60999580
· Oct 19, 2007
Provisional Application
60999575
· Oct 19, 2007