Fluorescence detection of poison oak oil
View Patent ↗The invention herein disclosed provides for compositions, methods for synthesizing said compositions, and methods for using said compositions, wherein the compositions and methods may be used to bind to and/or deactivate a poison oak oil, such as urushiol. The compositions and methods can be used to treat and/or reduce an inflammatory reaction and/or hypersensitivity to natural compounds found in poison oak, poison ivy, poison sumac, mango, lac tree, and cashew nut.
1. A method for detecting a catechol in a sample, the method comprising the steps of (i) contacting a boron composition and a nitroxide with the sample (ii) allowing the boron composition to react with the catechol in the sample thereby creating a catecholborane; (iii) allowing a first nitroxide to react with the catecholborane thereby generating an alkyl radical and a nitroxide-catecholborane complex; (iv) allowing the alkyl radical to react with a second nitroxide thereby creating an alkoxyamine; (v) measuring the amount of alkoxyamine, nitroxide-catecholborane complex, or an alkoxyamine hydrolysis product so created; the method resulting in detecting the catechol in the sample.
2. The method of claim 1 wherein the boron composition comprises a hydrophobic alkyl group.
3. The method of claim 1 wherein the catecholborane is a B-alkyl catecholborane.
4. The method of claim 2 wherein the alkyl group is selected from the group consisting of a hydrophobic alkyl group and a hydrophilic alkyl group.
5. The method of claim 1 wherein the nitroxide is a profluorescent nitroxide.
6. The method of claim 1 wherein the nitroxide is tetramethylpiperidinyloxy (TEMPO).
7. The method of claim 5 wherein the profluorescent nitroxide is dansyl amino-TEMPO.
8. The method of claim 1 wherein the nitroxide further comprises a fluorescent compound, the fluorescent compound selected from the group consisting of a hydrophobic fluorescent organic molecule, a hydrophilic fluorescent organic molecule, and a fluorescent quantum-dot nanoparticle.
9. The method of claim 1 wherein the measuring of the amount of alkoxyamine so created is performed using a photon source that results in fluorescence of the alkoxyamine and the nitroxide-catecholborane complex, wherein the fluorescence is visible to the naked eye.
10. The method of claim 1 wherein the measuring of the amount of alkoxyamine so created is performed using a photon source that induces fluorescence of the alkoxyamine and the nitroxide-catecholborane complex, wherein the fluorescence is detected by a photometer.
11. The method of claim 10 wherein the fluorescence comprises photons having a wavelength of between about 250 and 600 nm.
12. The method of claim 10 wherein the measuring further comprises measuring hydroxylamine complexed with boron or free hydroxylamine created by hydrolysis.
13. The method of claim 1 wherein the catechol is selected from the group consisting of urushiol, catechin, epicatechin, gallocatechin, epigallocatechin, epigallocatechin-3-gallate, and catecholamines epinephrine, norepinephrine, dopamine, and dihydroxyphenylalanine (DOPA).
14. The method of claim 13 , wherein the catechol is urushiol.
15. The method of claim 1 wherein the sample is selected from the group consisting of an area of a subject's skin, clothing, boots, pets, camping gear, tools, and other outdoor equipment.
16. The method of claim 1 wherein the sample is selected from the group consisting of a plant tissue, a plant extract, a plant tissue extract, an animal tissue, an animal extract, an animal tissue extract, and an animal fluid.
17. The method of claim 16 wherein the plant tissue is from a plant selected from the group consisting of poison oak, poison ivy, poison sumac, mango, cashew nut, and lac tree.
18. The method of claim 1 , further comprising the step of reacting the alkyl radical with a profluorescent nitroxide having a fluorescent tag, wherein the fluorescent tag is selected from the group consisting of an organic fluorophore and Cd—Se nanoparticle.
19. The method of claim 1 further comprising the step of measuring the amount of the nitroxide-catecholborane complex.
20. The method of claim 1 further comprising the step of measuring the amount of hydroxylamine hydrolysis product.
21. The method of claim 1 further comprising the step of measuring the amount of alkoxyamine product.