Anti-PCSK9 and methods for treating lipid and cholesterol disorders
View Patent ↗The present invention provides compositions and methods for treating disorders of cholesterol and lipid metabolism by administration of an anti-PCSK9 antibody or a peptide inhibitor of PCSK9.
1. An isolated antibody or antigen binding fragment thereof, which binds to a PCSK9 protein, comprising:
(a) CDR-H1 (SEQ ID NO: 51), CDR-H2 (SEQ ID NO: 52) and CDR-H3 (SEQ ID NO: 53) of an immunoglobulin heavy chain variable region which comprises the amino acid sequence of SEQ ID NO: 50; and
(b) CDR-L1 (SEQ ID NO: 55), CDR-L2 (SEQ ID NO: 56) and CDR-L3 (SEQ ID NO: 57) of an immunoglobulin light chain variable region which comprises the amino acid sequence of SEQ ID NO: 54.
2. The antibody or antigen-binding fragment thereof of claim 1 , wherein the antibody is selected from the group consisting of: a monoclonal antibody, a labeled antibody, a bivalent antibody, a bispecific antibody, a chimeric antibody, a recombinant antibody, a humanized antibody or a bispecific antibody.
3. The antibody or antigen binding fragment thereof of claim 1 , wherein the antigen binding fragment is selected from the group consisting of: an scfv, an scfv dimer, a dsfv, a (dsfv)2, a dsFv-dsfv′, a bispecific ds diabody, an Fv, an Fab′, an Fab′, and an F(ab′)2.
4. The antigen binding fragment of claim 1 which is linked to an immunoglobulin constant region polypeptide.
5. The polypeptide of claim 4 wherein the constant region polypeptide is a κ light chain, γ1 heavy chain, γ2 heavy chain, γ3 heavy chain or γ4 heavy chain.
6. A composition comprising the antibody or antigen binding fragment of claim 1 in association with a chemotherapeutic agent.
7. The composition of claim 6 , wherein the chemotherapeutic agent is selected from the group consisting of: a cardiovascular agent, an adrenergic blocker, an antihypertensive agent, an angiotensin system inhibitor, an angiotensin-converting enzyme inhibitor, a coronary vasodilator, a diuretic, an adrenergic stimulant, and an HMG-CoA reductase inhibitor.
8. The composition of claim 7 , wherein the chemotherapeutic agent a cardiovascular agent.
9. The composition of claim 6 , wherein the chemotherapeutic agent is selected from the group consisting of ezetimibe and simvastatin.