Process for Producing Particles Loaded with Growth Factors as Well as the Particles Thus Obtained
The present invention refers to a process for preparing a particulate material (particles) being loaded with growth factors, the thus obtained particles and the use for improving the ingrowth of implant material into the bone substance.
1 . Process for preparing a particulate inorganic material loaded with growth factors, wherein said particulate material being selected from ceramic materials is treated with growth factors in aqueous buffered solution in the acidic range between pH 4 and 5 over a period of at least 30 minutes, then the particulate material is separated from the aqueous buffered solution and washed at least once with the same volume of the buffered solution free of growth factor.
2 . Process according to claim 1 , wherein said particulate material is dried after the washing step with the aqueous buffered solution free of growth factor.
3 . Process according to claim 2 , wherein said drying step comprises a lyophilisation.
4 . Process according to claim 3 , wherein said aqueous buffered solution has a pH-value of 4.3 to 4.7.
5 . Process according to claim 4 , wherein said particulate material has a particle size in the range of 10 to 500 μm and an interconnecting pore structure.
6 . Process according to claim 5 , wherein said particulate material is consisting of hydroxyl apatite, tricalcium phosphate, calcium carbonate, aluminum oxide or mixtures thereof.
7 . Process according to claim 1 , wherein a particulate material with a chemically activated surface is used.
8 . Process according to claim 7 , wherein said particulate material having a chemically activated surface is obtained by treating the particulate material with an activation agent.
9 . Process according to claim 7 , wherein BMP-2 or BMP-7 is used as growth factor.
10 . Particulate material obtainable according to the process of claim 1 .
11 . Use of the particulate material of claim 10 for preparing a pharmaceutical composition for promoting bone growth.
12 . Process according to claim 1 , wherein said aqueous buffered solution has a pH-value of 4.3 to 4.7.
13 . Process according to claim 1 , wherein said aqueous buffered solution has a pH-value of 4.5.
14 . Process according to claim 1 , wherein said particulate material has a particle size in the range of 10 to 500 μm and an interconnecting pore structure.
15 . Process according to claim 1 , wherein said particulate material is consisting of hydroxyl apatite, tricalcium phosphate, calcium carbonate, aluminum oxide or mixtures thereof.
16 . Process according to claim 1 , wherein said particulate material having a chemically activated surface is obtained by treating the particulate material with an activation agent selected from the group of silanes.
17 . Process according to claim 1 , wherein at least one of BMP-2 and BMP-7 is used as growth factor