Colorimetric test for antimalarial artemesin derivatives
View Patent ↗A process for testing a composition as containing an artemisinin derivative is provided that includes contacting the composition with a reagent made up of a hydrogen bonding polar organic solvent and an acid having a pK value of less than 3.8 at 25° Celsius and capable of acid catalyzing a decomposition reaction of the artemisinin derivative so as to provide a reaction mixture. The reaction mixture is allowed sufficient time at a reaction temperature for the artemisinin derivative to decompose to yield a colored decomposition product discerned by a normal unaided human eye. A kit for testing a composition for an artemisinin derivative according to the process is provided together with instructions for contacting the solvent and the acid with the composition to decompose the artemisinin derivative to yield the colored decomposition.
1. A process for testing a composition for the presence of an artemisinin derivatives comprising:
contacting the composition with a reagent, said reagent consisting of a hydrogen bonding polar organic solvent and an acid having a pK value of less than 3.8 at 25° Celsius and catalyzing acid decomposition of the artemisinin derivative; and
allowing sufficient time at a reaction temperature for the artemisinin derivative to decompose to yield a colored decomposition product discernable by a normal unaided human eye.
2. The process of claim 1 wherein said colored decomposition product is green.
3. The process of claim 2 wherein said colored decomposition product has a visual intensity that is proportional to concentration.
4. The process of claim 1 wherein the composition comprises the artemisinin derivative and at least one component of tablet adjuvants, antimicrobials, non-artemisinin derivative antimalarials and nonsteroidal anti-inflammatories.
5. The process wherein said hydrogen bonding polar organic solvent is methanol, ethanol, methylcellusolve, acetonitrile, acetic acid, acetone, or a combination thereof.
6. The process of claim 1 wherein said hydrogen bonding polar organic solvent is methanol.
7. The process of claim 1 wherein said hydrogen bonding organic solvent is acetic acid or phosphoric acid.
8. The process of claim 1 wherein the reaction temperature of the reaction mixture is between 30 and 40° Celsius and a boiling temperature of said hydrogen bonding polar organic solvent.
9. The process of claim 8 wherein the reaction temperature is between 30 and 40° Celsius and the reaction time is from 2 to 15 minutes.
10. The process of claim 1 further comprising abrading the composition from an oral dosage.
11. The process of claim 1 wherein contact between the composition and said reagent is through dissolution of the composition in said reagent.
12. The process of claim 1 wherein the composition comprises artesunate and further comprising contacting a second portion of the composition with water and a pH indicator showing a color change at pH between 3.5 and 4.0.