Minimally invasive assessment of IgE mediated allergy
View Patent ↗A system and method for determining the presence and level of allergy indicators in a human fluid sample such as, but not limited to, blood serum and saliva, is disclosed. In another embodiment, the method may assess a level of allergens in a consumable product. The system and method may make use of functionalized magnetic nanoparticles that have modified surfaces suitable for attracting allergy indicators from human fluid sample and allergens from consumable products. The system and method may provide a minimally invasive assessment of allergy indicators to determine whether one is allergic to a substance.
1. A method for determining a presence of an allergy indicator in a fluid, comprising:
providing at least one magnetic particle with a coating that allows for the capture of the allergy indicator in the fluid;
coating the at least one magnetic particle with an allergen;
coating the at least one magnetic particle with the allergy indicator by placing the at least one magnetic particle into the fluid where the at least one magnetic particle attracts the allergy indicator;
removing the at least one magnetic particle from the fluid after a period of time;
exposing the at least one magnetic particle to an anti-allergy indicator in a solution to separate the allergy indicator from the at least one magnetic particle;
analyzing the solution to determine absorbance of the solution to determine the presence of the allergy indicator; and
wherein coating the at least one magnetic particle with an allergen comprises coating the at least one magnetic particle with a charged polymer having a surface charge that is opposite to a surface charge of the at least one allergy indicator.
2. The method of claim 1 , wherein providing the at least one magnetic particle with a coating that allows for the capture of the allergy indicator in the fluid, further comprises synthesizing iron oxide particles.
3. The method of claim 2 , wherein providing the at least one magnetic particle with a coating that allows for the capture of the allergy indicator in the fluid, further comprises synthesizing magnetic particles by a chemical method that reduces chemical salts to nanoparticles of at least one substance.
4. The method of claim 3 , wherein providing the at least one magnetic particle with a coating that allows for the capture of the allergy indicator in the fluid, further comprises sonicating the solution, peptizing the magnetic particles with an acid, washing the magnetic particles, filtering the magnetic particles, and drying the magnetic particles.
5. The method of claim 1 , wherein coating the at least one magnetic particle with an allergen comprises coating the at least one magnetic particle with a positively charge polymer.
6. The method of claim 1 , wherein coating the at least one magnetic particle with the allergy indicator by placing the at least one magnetic particle into the fluid where the at least one magnetic particle attracts the allergy indicator comprises coating the at least one magnetic particle with a peanut extract.
7. The method of claim 1 , wherein removing the at least one magnetic particle from the fluid after a period of time comprises removing the at least one magnetic particle with a magnet.
8. The method of claim 1 , wherein removing the at least one magnetic particle from the fluid after a period of time comprises removing the at least one magnetic particle from the fluid after about two hours.
9. The method of claim 1 , wherein exposing the at least one magnetic particle to an anti-allergy indicator in a solution to separate the allergy indicator from the at least one magnetic particle comprises exposing the at least one magnetic particle to an anti-allergy indicator for between about five minutes and about one hour.
10. The method of claim 1 , wherein analyzing the solution to determine absorbance of the solution comprises reading the absorbance at 405 nm in a plate reader.
11. The method of claim 1 , wherein removing the at least at least one magnetic particle from the fluid after a period of time comprises removing the at least one magnetic particle with a magnet.
12. The method of claim 1 , wherein enhancing surface properties of the at least one magnetic particle comprises washing with acidic or basic media to expose surface charges on the at least one magnetic particle.