Microparticle-based transfection and activation of dendritic cells
The present invention provides an effective method for the transfection of dendritic cells by non-viral methods. The present invention provides this benefit by incubating dendritic cells and a specified transfection agent. The transfection agent comprises a polynucleotide and microparticles, with the microparticles being comprised of biodegradable polymer and cationic detergent. The dendritic cells and transfection agent are incubated for a time sufficient to transfect the dendritic cells with the polynucleotide.
1. A method for producing an immune response in a vertebrate subject in need thereof comprising:
activating T cells by subjecting them to dendritic cells produced by a method that comprises incubating the dendritic cells and a transfection agent for a time sufficient to transfect the dendritic cells with the polynucleotide, said transfection agent comprising a polynucleotide that encodes an antigen associated with a virus or a tumor adsorbed on microparticles comprised of a biodegradable polymer and a cationic detergent, wherein polynucleotide is not entrapped within said microparticles;
administering said activated T cells to said subject.
2. The method according to claim 1 , in which the dendritic cells and T cells originate from the vertebrate subject.
3. The method according to claim 1 , in which the dendritic cells and T cells originate from a healthy vertebrate subject MHC-matched to the vertebrate subject.
4. The method according to claim 1 , in which the T cells are administered parenterally.
5. The method according to claim 1 , in which the T cells are administered by direct injection into affected tissue.