OMP85 Proteins of Neisseria Gonorrhoeae and Neisseria Meningitidis, Compositions Containing Same and Methods of Use Thereof
Nucleic acid and amino acid sequences of the Omp85 proteins of N. gonorrhoeae and N. meningitidis , and fragments thereof are useful in vaccine compositions, therapeutic compositions and diagnostic compositions for use in the prevention, treatment and diagnosis of non-symptomatic gonococcal infection or symptomatic disease and non-symptomatic meningococcal infection and symptomatic disease. Antibodies are developed to these proteins and also useful in the compositions and methods described herein.
1 . A method of producing an immunogenic composition comprising isolating a recombinant polypeptide comprising an epitope of at least 8 consecutive amino acids within the amino acid sequence of SEQ ID NO: 4, and providing said polypeptide with a pharmaceutically acceptable carrier, wherein said composition induces antibodies in a mammal that bind to said epitope of SEQ ID NO: 4 and that interfere with adherence of Neisseria gonorrhoeae as measured by the gonococcal cell adherence assay.
2 . The method of claim 1 , wherein said composition comprises a second polypeptide or protein.
3 . The method according to claim 2 , wherein said second polypeptide or protein is an antigen from a pathogenic bacterial species that is heterologous or homologous to Neisseria gonorrhoeae or Neisseria meningitidis.
4 . The method according to claim 1 , wherein said composition further comprises an adjuvant.