Polyinosinic acid-polycytidylic acid-based adjuvant
The present invention provides a polynucleotide adjuvant composition and methods of use in eliciting an immune response. The present invention also provides an immunogenic composition comprising the polynucleotide adjuvant composition together with an antigen (e.g., as in a vaccine). The adjuvant compositions of the invention have particular physical properties (e.g., molecular weight, concentration, and pH) which address the need for a safe adjuvant for eliciting an enhanced immune response. The present invention further contemplates methods of use of such adjuvant compositions, particularly in eliciting an immune response to an antigenic compound.
1. An immunogenic composition, comprising:
a polynucleotide adjuvant composition comprising polynucleotide adjuvant composition molecules comprising a polyriboinosinic polyribocytidylic acid (PIC), an antibiotic, and a positive ion, and
an antigenic compound;
wherein the polynucleotide adjuvant composition molecules comprise molecules ranging from 9.0 Svedbergs to 12.0 Svedbergs or greater, and
the composition is suitable for use in humans.
2. The immunogenic composition of claim 1 ,
wherein the polynucleotide adjuvant composition molecules have an average molecular size greater than 12 Svedbergs.
3. The immunogenic composition of claim 1 or 2 , wherein the antigenic compound is a human antigen, a non-human animal antigen, a plant antigen, bacterial antigen, a fungal antigen, a viral antigen, a parasite antigen, or a cancer antigen.
4. The immunogenic composition of claim 1 or 2 , wherein the viral antigen is a rabies antigen.
5. The immunogenic composition of claim 4 , wherein the rabies antigen is an inactivated purified rabies antigen.
6. The immunogenic composition of claim 1 or 2 , wherein polynucleotide adjuvant composition is capable of eliciting an enhanced specific humoral and/or cell mediated immune response.
7. The immunogenic composition of claim 1 or 2 , wherein the adjuvant composition and/or the immunogenic composition is in a solid form or a liquid form, wherein the liquid form is a solution or a suspension.
8. The immunogenic composition of claim 1 or 2 , wherein at least one of the adjuvant composition or the immunogenic composition is freeze-dried.
9. A method for enhancing an immune response to an antigenic compound, comprising:
administering to a subject a composition comprising an antigenic compound and the polynucleotide adjuvant composition of claim 1 or 2 .
10. The method of claim 9 , wherein said administering is by parenteral injection, intramuscular injection, intraperitoneal injection, intravenous injection, subcutaneous injection, inhalation, rectal delivery, vaginal delivery, nasal delivery, oral delivery, ophthalmic delivery, topical delivery, transdermal delivery or intradermal delivery.