Delivery of DNA vaccines into fish by immersion
A method for immunizing fish against pathogenic infection is disclosed. More particularly, this invention provides a method of immunizing fish against bacterial and viral infection which comprises immersing the fish in a solution comprised of an effective amount of dead or attenuated, whole-celled recombinant bacteria, the recombinant bacteria having a eukaryotic expression vector (plasmid) incorporated therein having DNA of interest encoding at least one protein antigen for each of the pathogens, the method characterized in that the protein antigen is produced by the fish.
1. A method of inducing an immune response in a fish against one or more pathogens which comprises:
transforming a bacterium consisting of a live, attenuated strain of V. anguillarum which comprises a mugA gene comprising nucleotides 1218-2610 of SEQ ID NO: 5, the strain having a mutation located within nucleotides 1218-2610 of SEQ ID NO: 5 that renders the strain incapable of expressing a functional mugA protein, with a plasmid comprising DNA of interest encoding at least one protein antigen for each of the pathogens; and;
immersing the fish in a solution comprised of the, the method characterized in that the protein antigen is produced by the fish.
2. The method according to claim 1 wherein the fish is selected from the group of finfish.
3. The method according to claim 1 wherein the plasmid comprises a fish promoter, a polyadenylation signal of fish origin and a kanamycin resistance cassette.
4. The method according to claim 3 wherein the polyadenylation signal is wolfish AFP poly A.