Polypeptides having endoglucanase activity and polynucleotides encoding same
The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
1. An isolated polypeptide having endoglucanase activity, selected from the group consisting of:
(a) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 4;
(b) a polypeptide encoded by a polynucleotide that hybridizes under high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 3, (ii) the genomic DNA sequence comprising the mature polypeptide coding sequence of SEQ ID NO: 3, or (iii) a full-length complementary strand of (i) or (ii), wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide for 12 to 24 hours and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.; and
(c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 3.
2. The polypeptide of claim 1 , comprising an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 4.
3. The polypeptide of claim 2 , comprising an amino acid sequence having at least 97% sequence identity to the mature polypeptide of SEQ ID NO: 4.
4. The polypeptide of claim 1 , comprising or consisting of the amino acid sequence of SEQ ID NO: 4; or a fragment thereof having endoglucanase activity.
5. The polypeptide of claim 4 , comprising or consisting of the amino acid sequence of SEQ ID NO: 4.
6. The polypeptide of claim 1 , comprising or consisting of the mature polypeptide of SEQ ID NO: 4.
7. The polypeptide of claim 1 , which is encoded by a polynucleotide that hybridizes under high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 3, (ii) the genomic DNA sequence comprising the mature polypeptide coding sequence of SEQ ID NO: 3, or (iii) a full-length complementary strand of (i) or (ii), wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide for 12 to 24 hours and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.
8. The polypeptide of claim 7 , which is encoded by a polynucleotide that hybridizes under very high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 3, (ii) the genomic DNA sequence comprising the mature polypeptide coding sequence of SEQ ID NO: 3, or (iii) a full-length complementary strand of (i) or (ii), wherein very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide for 12 to 24 hours and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 70° C.
9. The polypeptide of claim 1 , which is encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 3.
10. The polypeptide of claim 9 , which is encoded by a polynucleotide comprising a nucleotide sequence having at least 97% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 3.
11. The polypeptide of claim 1 , which is encoded by a polynucleotide comprising or consisting of the nucleotide sequence of SEQ ID NO: 3; or a subsequence thereof encoding a fragment having endoglucanase activity.
12. The polypeptide of claim 11 , which is encoded by a polynucleotide comprising or consisting of the nucleotide sequence of SEQ ID NO: 3.
13. The polypeptide of claim 1 , which is encoded by a polynucleotide comprising or consisting of the mature polypeptide coding sequence of SEQ ID NO: 3.
14. The polypeptide of claim 1 , which is encoded by the polynucleotide contained in plasmid pTter16B which is contained in E. coli NRRL B-50082.
15. The polypeptide of claim 1 , wherein the mature polypeptide is amino acids 19 to 288 of SEQ ID NO: 4.
16. The polypeptide of claim 1 , wherein the mature polypeptide coding sequence is nucleotides 55 to 855 of SEQ ID NO: 3.