Tissue specific promoters
The invention relates to tissue specific promoters which can be used in plants for one or more of the following purposes: a. altering carbohydrate metabolism b. preventing memory substance loss c. expression of an invertase inhibitor d. expression of a fructosyl transferase e. expression of a levan sucrase f. expression of genes coding for transported proteins for N-compounds g. expression of characteristics which increase resistance/tolerance with respect to pathogens.
1. An isolated promoter comprising a nucleotide sequence selected from the group consisting of:
a. SEQ ID NO: 2; and
b. the polynucleotide sequence complementary to the full length of SEQ ID NO: 2.
2. The isolated promoter of claim 1 inserted into a vector or operably connected to a mobile genetic element.
3. A transgenic eukaryotic or a transgenic prokaryotic host cell comprising a promoter that is operably linked to a transgene, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
a. SEQ ID NO: 2 having a promoter activity; and
b. the polynucleotide sequence complementary to the full length of SEQ ID NO: 2.
4. A transgenic plant comprising a promoter that is operably linked to a transgene, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
a. SEQ ID NO: 2 having a promoter activity; and
b. the polynucleotide sequence complementary to the full length of SEQ ID NO: 2.
5. The transgenic plant according to claim 4 wherein said transgenic plant is Beta vulgaris , and wherein said promoter is active in the root, but not the above-ground organs, of Beta vulgaris.
6. The transgenic plant according to claim 4 , wherein said transgenic plant is further characterized by the expression of a transgene exclusively in a below-ground organ, wherein said expression is obtainable by transformation of a plant cell with said promoter that is operably linked to a transgene.
7. A transgenic seed produced by said transgenic plant of claim 4 .
8. The transgenic plant according to claim 6 , wherein said below-ground organ is a root.
9. The transgenic plant according to claim 6 , wherein said plant belongs to species Beta vulgaris , and wherein said promoter is active in the root, but not the above-ground organs, of Beta vulgaris.
10. The transgenic plant according to claim 6 , wherein said transgenic plant is characterized by:
a. amending a carbohydrate metabolism;
b. avoiding a loss of a storage substance;
c. expressing an invertase inhibitor;
d. expressing a fructosyl transferase;
e. expressing a levan sucrose;
f. expressing a gene coding for a transporter protein for an N-compound; or
g. developing a feature that increases at least one of resistance and tolerance towards pathogens.
11. A method of:
a. amending carbohydrate metabolism;
b. avoiding loss of storage substance;
c. increasing at least one of resistance and tolerance towards a pathogen; or
d. expressing from a transgene:
an invertase inhibitor,
a fructosyl transferase,
a levan sucrose, or
a transporter protein from an N-compound, in a transgenic plant,
said method comprising:
(1) transforming a plant cell with a promoter, wherein said promoter is operably linked to said transgene; and
(2) subsequently regenerating a transgenic plant from said plant cell, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
i. SEQ ID NO: 2; and
ii. the polynucleotide sequence complementary to SEQ ID NO: 2.