Biosensor strip
The present invention discloses a biosensor strip, which comprises: a base plate layer defining a first strip end and a second strip end; a conductive layer being disposed on the base plate layer and partitioned into at least two electrode paths; a reagent containing layer being disposed on the conductive layer and comprising a first through hole that is located at the first strip end and for accommodating a reagent solution, wherein the reagent solution comprises matrix, redox mediator, enzyme, surfactant, and a buffer solution; a channel forming layer being disposed on the reagent containing layer and comprising a gap portion that is located at the first strip end; and a cover layer being disposed on the channel forming layer and comprising a second through hole that exposes the partial area of the gap portion of the channel forming layer.
1. A biosensor strip comprising:
a base plate layer defining a first strip end and a second strip end;
a conductive layer being disposed on the base plate layer and partitioned into a first electrode path, a second electrode path and a third second electrode path, wherein the three electrode paths are insulated from each other and the conductive layer comprises a conductive contact at the second strip end;
a reagent containing layer being disposed on the conductive layer and comprising a first through hole that is located near the first strip end and for accommodating a reagent solution, wherein the first through hole exposes a partial area of the three electrode paths simultaneously and the reagent solution comprises matrix, redox mediator, enzyme, surfactant, and a buffer solution;
a channel forming layer being disposed on the reagent containing layer and comprising a gap portion that is located at the first strip end, wherein the gap portion exposes the first through hole; and
a cover layer being disposed on the channel forming layer and comprising a second through hole that exposes a partial area of the gap portion of the channel forming layer;
an inlet notch located in the cover layer at the first strip end for indicating an entrance of a sample channel; and
at least one scribe line perpendicular to a direction connecting said first strip end and said second strip end, said at least one scribe line being configured for adjusting the area ratio of the first electrode path and the second electrode path exposed in the first through hole of the reagent containing layer;
wherein a sample channel is formed with the gap portion, the cover layer, the first and second through holes and the conductive layer by placing the channel forming layer between the reagent containing layer and the cover layer and the second through hole is as a vent opening so as to increase the speed of a fluid sample getting into the sample channel;
wherein the first electrode path is a working electrode and the second electrode path is a counter electrode, and third electrode path further be a short-fill electrode which is used to ensure that the fluid sample get into the sample channel completely;
wherein after the short-fill electrode receives the signals of the current changes, the working electrode and the counter electrode carry out their works.
2. The biosensor strip according to claim 1 , wherein the conductive layer, the reagent containing layer, the channel forming layer, and the cover layer are combined tightly to each other by a thin layer of colloid.
3. The biosensor strip according to claim 1 , wherein the base plate layer, the reagent containing layer and the channel forming layer are made of an insulated material and the cover layer is made of an insulated and hydrophilic material.