Method for making nanolipidic particles
Nanolipidic Particles (NLPs) having average mean diameters of 1 nm to 20 nm are made from a precursor solution. NLPs can be loaded with a desired passenger molecule. Assemblies of these particles, called NLP assemblies, result in a vehicle population of a desired size. Single application or multifunction NLP assemblies are made from the loaded NLPs and range in size from about 30 to about 200 nm. A method of using preloaded NLPs to make larger carrier vehicles or a mixed population provides increased encapsulation efficiency. NLPs have application in the cosmetics, pharmaceutical, and food and beverage industries.
1. A method for making an NLP (Nanolipidic Particle) assembly population, comprising the steps of:
(a) diluting a monophasic optically clear precursor solution with an ethanol solvent to form solvent diluted stock solution; wherein said diluting of said precursor solution with said ethanol solvent is at a ratio ranging from about 1 part precursor to about 20 parts solvent to a ratio ranging from about 1 part precursor to about 0.3 parts solvent; and
(b) adding an aqueous solution comprising a water soluble passenger molecule to said solvent diluted stock solution, whereby an NLP assembly population is formed having a mean particle diameter from about 30 to 200 nm; and wherein the concentration of said solvent in said NLPs is from about 0.5% to about 14%.
2. The method of claim 1 , wherein said diluting of said precursor solution with said ethanol solvent is at a ratio of about 1 part precursor to about 10 parts solvent to about 1 part loaded vehicle population to about 0.5 parts solvent.
3. The method of claim 1 , wherein said precursor solution comprises phospholipids selected from the group consisting of phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA) and phosphatidylinositol (PI) and mixtures thereof.
4. The method of claim 1 , wherein said phospholipids consist of a mixture of phosphatidylethanolamine (PE), phosphatidic acid (PA) and phosphatidylinositol (PI).
5. The method of claim 3 , wherein said phospholipids consist of a mixture of phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA) and phosphatidylinositol (PI).
6. The method of claim 5 , wherein the ratio of said phospholipids in said mixture is PC:PE:PA:PI of 6.5:2.5:0.7:0.3.
7. The method of claim 1 , comprising making one or more additional dilutions of said precursor solution with said ethanol solvent in order to provide a desired size of NLPs and number of NLPs per unit volume.
8. The method of claim 1 , wherein said water-soluble passenger molecule is a water-soluble vitamin.
9. The method of claim 8 , wherein said water-soluble vitamin is Vitamin C.