IP Library › Patent Application 12773320
Patent Application
App. No. 12/773,320

RECOMBINANT HOST FOR PRODUCING L-ASPARAGINASE II

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Quick Facts
Patent No.
US None
App. No.
12/773,320
Abstract

The invention provides a recombinant Escherichia coli host cell for producing an Escherichia coli L-asparaginase II enzyme. The host cell includes an Escherichia coli chromosome and at least one copy of a recombinant extrachromosomal vector, wherein the recombinant extrachromosomal vector encodes the L-asparaginase II enzyme, wherein the host cell chromosome also encodes the same L-asparaginase II enzyme, and wherein the host chromosome does not encode any other isoform of L-asparaginase II.

Claims (6)

1 . A recombinant Escherichia coli host cell for producing an Escherichia coli L-asparaginase II enzyme, comprising an Escherichia coli host cell chromosome and at least one copy of a recombinant extrachromosomal vector, wherein the recombinant extrachromosomal vector encodes a subunit of the L-asparaginase II enzyme, wherein the host cell chromosome also encodes the same subunit of the L-asparaginase II enzyme, and wherein the host cell chromosome does not encode any other isoform of L-asparaginase II, wherein the encoded L-asparaginase II subunit comprises SEQ ID NO: 1.

2 . The recombinant Escherichia coli host cell of claim 1 , wherein the extrachromosomal vector is a plasmid.

3 . The recombinant Escherichia coli host cell of claim 1 wherein the recombinant extrachromosomal vector comprises a DNA molecule encoding the L-asparaginase protein, that is operatively connected to a suitable promoter.

4 . The recombinant Escherichia coli host cell of claim 3 wherein the promoter is selected from the group consisting of T7, araB, P R /P L , phoA, trc, and trp promoters.

5 . The recombinant Escherichia coli host cell of claim 3 wherein the recombinant extrachromosomal vector further comprises an operator, ribosome binding site, signal sequence, transcriptional terminator, antibiotic selection marker, origin of replication, and a regulated copy of the repressor.

6 . A method of producing a recombinant L-asparaginase II enzyme substantially free of other L-asparaginase II isomers, comprising culturing the host cell of claim 1 , and isolating the produced L-asparaginase II enzyme.

Assignments (2)
MERGER Recorded Apr 29, 2015
From: SIGMA TAU RARE DISEASES S.A.
To: SIGMA-TAU PHARMA LIMITED
Reel/Frame 035551/0219 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Feb 7, 2012
From: DEFIANTE FARMACEUTICA, S.A.
To: SIGMA-TAU RARE DISEASES, S.A.
Reel/Frame 027662/0812 →