Compositions and methods for pest control
Provided herein are compositions and kits comprising carbohydrate-based inhibitors that bind GNBP and/or inhibit β(1,3)-glucanase activity. Also provided are methods of using such inhibitors to protect against or treat pest infestation, as are compositions and kits comprising subparts of the carbohydrate-based inhibitors.
1. A method for providing protection against or treating a pest infestation consisting of:
contacting a pest, soil, wood, plant, seeds, grain or manmade structure with a carbohydrate-based inhibitor that inhibits β(1,3)-glucanase activity of gram-negative bacteria binding protein (GNBP) in an amount effective to protect against or to treat the pest infestation,
wherein the carbohydrate-based inhibitor does not inhibit cellulase or stimulate feeding in the pests of the pest infestation.
2. The method of claim 1 , wherein the carbohydrate-based inhibitor does not inhibit α and/or β-1,4-glucosidase in the pests of the pest infestation.
3. The method of claim 1 , wherein carbohydrate-based inhibitor inhibits β(1,3)-glucanase activity and not the activity of another glucanase and/or glucosidase in the pests of the pest infestation.
4. The method of claim 1 , wherein the carbohydrate-based inhibitor is not gluconolactone.
5. The method of claim 1 , wherein the carbohydrate-based inhibitor comprises a β(1,3) glycosidic linkage at a reducing end.
6. The method of claim 1 , wherein the carbohydrate-based inhibitor is conjugated to a nanoparticle.
7. The method of claim 1 , wherein the carbohydrate-based inhibitor is conjugated to a dendrimer.
8. The method of claim 1 , wherein the carbohydrate-based inhibitor is conjugated to polyacrylamide.
9. The method of claim 1 , wherein the carbohydrate-based inhibitor is a di/tri/tetrameric β(1,3)-D-glucan derivative or a di/tri/tetrameric β(1,3)-L-glucan derivative.
10. The method of claim 9 , wherein the di/tri/tetrameric β(1,3)-D-glucan derivative or di/tri/tetrameric β(1,3)-L-glucan derivative is D-Glc-β(1,3)-D-glucono-Δ-lactone, D-Glc-β(1,3)-D-Glc-β(1,3)-D-Glc-β(1,3)-D-glucono-Δ-lactone, L-Glc-β(1,3)-L-glucono-Δ-lactone or L-Glc-β(1,3)-L-Glc-β(1,3)-L-Glc-β(1,3)-L-glucono-Δ-lactone.
11. The method of claim 1 , wherein the carbohydrate-based inhibitor is a β(1,3)-D-glucan-LPS (lipopolysaccharide) conjugate or a β(1,3)-L-glucan-LPS conjugate.
12. The method of claim 11 , wherein the β(1,3)-D-glucan-LPS conjugate is eritoran-D-glucono-Δ-lactone.
13. The method of claim 11 , wherein the β(1,3)-L-glucan-LPS conjugate is eritoran-L-glucono-Δ-lactone.
14. The method of claim 11 , wherein the β(1,3)-D-glucan or β(1,3)-L-glucan is conjugated to the LPS with an acyl spacer.
15. The method of claim 11 , wherein the β(1,3)-D-glucan or β(1,3)-L-glucan is conjugated to the LPS with a heterobifunctional cross-linker.
16. The method of claim 1 , wherein the carbohydrate-based inhibitor is a polyvalent D-glucono-D-lactone-LPS conjugate or a polyvalent L-glucono-L-lactone-LPS conjugate.
17. The method of claim 16 , wherein the polyvalent D-glucono-D-lactone-LPS conjugate or a polyvalent L-glucono-L-lactone-LPS conjugate is conjugated to a nanoparticle.
18. The method of claim 16 , wherein the polyvalent D-glucono-D-lactone-LPS conjugate or a polyvalent L-glucono-L-lactone-LPS conjugate is conjugated to a dendrimer.
19. The method of claim 16 , wherein the polyvalent D-glucono-D-lactone-LPS conjugate or a polyvalent L-glucono-L-lactone-LPS conjugate is conjugated to polyacrylamide.
20. The method of claim 16 , wherein the polyvalent D-glucono-D-lactone or polyvalent L-glucono-L-lactone is D-δ-gluconolactone (GDL).
21. The method of claim 16 , wherein the LPS is eritoran.
22. The method of claim 1 , wherein the GNBP is GNBP-1 or GNBP-2.
23. The method of claim 22 , wherein the GNBP is GNBP-2.
24. The method of claim 1 , wherein the pest infestation is an insect infestation.
25. The method of claim 1 , wherein the pest infestation is a plant pest infestation.
26. The method of claim 1 , wherein the pest infestation is a/an termite, fly, moth, ant, beetle, mosquito, silk worm, locust or cockroach infestation.
27. The method of claim 26 , wherein the pest infestation is a termite infestation.
28. The method of claim 27 , wherein the termites of the termite infestation are of the N. corniger, Z. augusticollis, C. secundus, R. virginicus or R. flavipes species.
29. The method of claim 1 , wherein the contacting comprises applying or spraying the carbohydrate-based inhibitor onto the pest, soil, wood, plant, seeds, grain or manmade structure.
30. The method of claim 1 , wherein the contacting comprises soaking the pest, soil, wood, plant, seeds, grain or manmade structure with the carbohydrate-based inhibitor.
31. The method of claim 1 , wherein the contacting comprises incorporating or impregnating the carbohydrate-based inhibitor into the pest, soil, wood, plant, seeds, grain or manmade structure.
32. The method of claim 1 , wherein the method further comprises contacting the pest, soil, wood, plant, seeds, grain or manmade structure with another pesticide.
33. The method of claim 32 , wherein the other pesticide is an insecticide, fungicide or a herbicide.
34. The method of claim 1 , wherein the carbohydrate-based inhibitor is in an aqueous composition.
35. The method of claim 1 , wherein the carbohydrate-based inhibitor is in a dry formulation.