Cell Therapy for Brain Tissue Damage
Disclosed are methods for conditioning stems cells and using the conditioned stems cells for treating brain tissue damage.
1 . A method of improving neurological behavior function of a subject having brain tissue damage, comprising
identifying a subject suffering from brain tissue damage, and
administering to the subject a composition containing an effective amount of a pluripotent cell,
wherein the pluripotent cell is prepared by a process comprising culturing the cell under a hypoxia condition.
2 . The method of claim 1 , wherein the pluripotent cell is a CD34 + cell.
3 . The method of claim 1 , wherein the pluripotent cell is a CD34 + cell and is obtained from umbilical cord blood.
4 . The method of claim 1 , wherein the process further comprises evaluating the Epac1 level in the cell after culturing the cell under a hypoxia condition.
5 . The method of claim 1 , wherein the composition is administered intracerebrally.
6 . The method of claim 1 , wherein the method further comprises evaluating a therapeutic effect on the subject by a non-invasive technique.
7 . The method of claim 1 , wherein culturing the cell under a hypoxia condition is conducted by placing the cell in a medium containing 60 to 600 mM Desferrioxamine (DFX) for 12 to 48 hours.
8 . The method of claim 7 , wherein culturing the cell under a hypoxia condition is conducted by placing the cell in a medium containing 100 to 450 mM Desferrioxamine (DFX) for 16 to 36 hours.
9 . The method of claim 8 , wherein culturing the cell under a hypoxia condition is conducted by placing the cell in a medium containing 200 to 350 mM Desferrioxamine (DFX) for 20 to 24 hours.
10 . The method of claim 1 , wherein culturing the cell under a hypoxia condition is conducted by placing the cell in an incubator containing 0.5 to 3% O 2 for 6 to 48 hours.
11 . The method of claim 10 , wherein culturing the cell under a hypoxia condition is conducted by placing the cell in an incubator containing 0.8 to 1.5% O 2 for 12 to 36 hours.
12 . The method of claim 11 , wherein culturing the cell under a hypoxia condition is conducted by placing the cell in an incubator containing 0.9 to 1.1% O 2 for 23 to 25 hours.
13 . A method of increasing angiogenesis in a tissue of a subject, comprising administering to a tissue of a subject in need thereof a composition containing an effective amount of a pluripotent cell, wherein the pluripotent cell is prepared by a process comprising culturing the cell under a hypoxia condition.
14 . The method of claim 13 , wherein the subject has brain tissue damage and the tissue is the brain of the subject.
15 . The method of claim 1 , wherein culturing the cell under a hypoxia condition is conducted by placing the cell in a medium containing 10 to 500 μM CoCl 2 for 12 to 48 hours.
16 . The method of claim 1 , wherein culturing the cell under a hypoxia condition is conducted by placing the cell in a medium containing about 100 μM CoCl 2 for 12 to 48 hours.
17 . A composition comprising one or more pluripotent cells and a hypoxia agent.
18 . The composition of claim 17 , wherein the one or more pluripotent cells are CD34+ cells.
19 . The composition of claim 18 , wherein the one or more pluripotent cells are obtained from umbilical cord blood.
20 . The composition of claim 19 , wherein the hypoxia agent is Desferrioxamine or CoCl 2 .