Isolated bitter taste receptor polypeptides
The claimed invention relates to the discovery of a specific human taste receptor in the T2R taste receptor family, hT2R61 that responds to particular bitter compounds The present invention further relates to the use of this receptor in assays for identifying ligands that modulate the activation of this taste receptor. These compounds may be used as additives and/or removed from foods, beverages and medicinals in order to modify (block) T2R-associated bitter taste. A preferred embodiment is the use of the identified compounds as additives in foods, beverages and medicinals for blocking bitter taste.
1. An isolated bitter taste receptor polypeptide that is at least 95% identical to the polypeptide in SEQ ID NO:8 or the extracellular or transmembrane domains thereof.
2. The isolated bitter taste receptor polypeptide of claim 1 that is at least 96% identical to the polypeptide in SEQ ID NO:8 or the extracellular or transmembrane domains thereof.
3. The isolated bitter taste receptor polypeptide of claim 1 that is at least 97% identical to the polypeptide in SEQ ID NO:8 or the extracellular or transmembrane domains thereof.
4. The isolated bitter taste receptor polypeptide of claim 1 that is at least 98% identical to the polypeptide in SEQ ID NO:8 or the extracellular or transmembrane domains thereof.
5. The isolated bitter taste receptor polypeptide of claim 1 that is at least 99% identical to the polypeptide in SEQ ID NO:8 or the extracellular or transmembrane domains thereof.
6. The isolated bitter taste receptor polypeptide of claim 1 that is at least identical to the polypeptide in SEQ ID NO:8 or the extracellular or transmembrane domains thereof.
7. A fusion protein comprising an isolated bitter taste receptor polypeptide according to claim 1 .
8. A fusion protein comprising an isolated bitter taste receptor polypeptide according to claim 5 .
9. A fusion protein according to claim 7 further comprising a portion of another taste receptor polypeptide or extracellular or transmembrane domain thereof.