HALOGENATED RHODAMINE DERIVATIVES AND APPLICATIONS THEREOF
Methods for treating a bacterial infection in a tissue sample are described. Tissue samples that are harvested from a patient may be infected with bacteria. A rhodmaine compound is mixed with the infected tissue sample to form a mixture. The mixture is then exposed to radiant energy to inhibit or kill the bacteria. The exposed mixture is then transplanted into the patient. Examples of rhodamine compounds include 2′-(6-dimethylamino-3-dimethylimino-3H-xanthen-9-yl) 4′, 5′-dichloro-benzoic acid methyl ester hydrochloride; 4,5-dibromorhodamine 110 2-(2-methoxy ethoxy)ethyl ester hydrobromide; acetate salt of 2,7-dibromorhodamine B hexyl ester; acetate salt of 2,7-dibromorhodamine B methyl ester; 4,5-dibromorhodamine 6G hydrobromide; rhodamine B 3-bromopropyl; acetate salt of 2,7-dibromo-4′-carboxytetramethylrosamine methyl ester; 4-bromo-5-phenyl rhodamine B methyl ester chloride; 2,7-dibromo-4,5-dimethyl rhodamine B methyl ester bromide; 2-bromo-7-ethynyl rhodamine B methyl ester bromide; and 4,5-dibromo-2,7-di-n-butyl rhodamine B methyl ester bromide.
1 . A method for treating a tissue sample from a patient, comprising the steps of:
harvesting the tissue sample from the patient, wherein the tissue sample is infected with bacteria;
providing a rhodamine compound selected from the group consisting of 2′-(6-dimethylamino-3-dimethylimino-3H-xanthen-9-yl) 4′,5′-dichloro-benzoic acid methyl ester hydrochloride; 4,5-dibromorhodamine 110 2-(2-methoxy ethoxy)ethyl ester hydrobromide; acetate salt of 2,7-dibromorhodamine B hexyl ester; acetate salt of 2,7-dibromorhodamine B methyl ester; 4,5-dibromorhodamine 6G hydrobromide; rhodamine B 3-bromopropyl; acetate salt of 2,7-dibromo-4′-carboxytetramethylrosamine methyl ester; 4-bromo-5-phenyl rhodamine B methyl ester chloride; 2,7-dibromo-4,5-dimethyl rhodamine B methyl ester bromide; 2-bromo-7-ethynyl rhodamine B methyl ester bromide; and 4,5-dibromo-2,7-di-n-butyl rhodamine B methyl ester bromide;
mixing the rhodamine compound and the tissue sample to form a mixture,
exposing the mixture to radiant energy to inhibit or kill the bacteria; and
transplanting the exposed mixture into the patient.
2 . The method of claim 1 , wherein the bacteria is gram positive.
3 . The method of claim 1 , wherein the bacteria is gram negative.
4 . The method of claim 1 , wherein the bacteria is Staphylococcus epidermitis.
5 . The method of claim 1 , wherein the bacteria is at least one of Escherichia coli 0157:H7 or Salmonella thyphimurium , and wherein the rhodamine compound is 4,5-dibromo rhodamine 110 2-(2-methoxy ethoxy)ethyl ester.
6 . The method of claim 1 , wherein the bacteria is Salmonella thyphimurium LT2, and wherein the rhodamine compound is the acetate salt of 2,7-dibromorhodamine B hexyl ester.
7 . The method of claim 1 , wherein the bacteria is Escherichia coli 0157:H7, and wherein the rhodamine compound is 4,5-dibromorhodamine 6G hydrobromide
8 . The method of claim 1 , wherein the bacteria is Escherichia coli 0157TH7, and wherein the compound is rhodamine B 3-bromopropyl ester.
9 . The method of claim 1 , wherein the bacteria is selected from the group consisting of Escherichia coli 0157TH7, Salmonella thyphimurium LT2, and Pseudomonas aeruginosa , and wherein the rhodamine compound is 4,5-dibromorhodamine methyl ester.
10 . The method of claim 1 , wherein the mixture is exposed to a wavelength of 514 nm.
11 . The method of claim 1 , wherein the tissue sample is blood.
12 . The method of claim 1 , wherein the tissue sample is bone marrow.
13 . The method of claim 1 , wherein the mixture is exposed to radiant energy for 180 minutes.
14 . The method of claim 1 , further comprising the steps of
centrifuging the mixture:
resuspending the centrifuged mixture; and
incubating the resuspended centrifuged mixture.
15 . The method of claim 14 , wherein the resuspended centrifuged mixture is incubated for 90 minutes.
16 . The method of claim 14 , wherein the resuspended centrifuged mixture is incubated before exposing the mixture to radiant energy.