Treatment of muscular dystrophies and related disorders
The invention provides, among other aspects, compositions and methods for treating, preventing, and diagnosing diseases or conditions associated with an abnormal level or activity of biglycan; diseases or conditions associated with an abnormal level or activity of collagen VI; disorders associated with an unstable cytoplasmic membrane, due, e.g., to an unstable dystrophin associated protein complex (DAPC); and disorders associated with abnormal synapses or neuromuscular junctions, including those resulting from an abnormal MuSK activation or acetylcholine receptor (AChR) aggregation.
1. A method for treating Bethlem myopathy, Ullrich Congenital Muscular Dystrophy or Sorsby's fundus dystrophy, comprising administering to a mammal a biglycan polypeptide, which biglycan polypeptide comprises an amino acid sequence that is at least about 90% identical to amino acids 38-368 of SEQ ID NO: 9, or a portion thereof, and which amino acid sequence has a biological activity of human biglycan.
2. The method of claim 1 , wherein the biglycan polypeptide binds to Muscle-specific kinase (MuSK) on a mammalian cell.
3. The method of claim 1 , wherein the biglycan polypeptide binds to a α-sarcoglycan and/or γ-sarcoglycan on a mammalian cell.
4. The method of claim 1 , wherein the biglycan polypeptide binds to a collagen VI polypeptide on a mammalian cell.
5. The method of claim 1 , wherein the biglycan polypeptide causes phosphorylation of sarcoglycans on a mammalian cell.
6. The method of claim 1 , wherein the biglycan polypeptide causes an increase in utrophin levels in a mammalian cell.
7. The method of claim 1 , wherein the biglycan polypeptide is derivatized with one or more glycosaminoglycan (GAG) side chains.
8. The method of claim 1 , wherein the amino acid sequence of the biglycan polypeptide is at least about 95% identical to amino acids 38-368 of SEQ ID NO: 9.
9. The method of claim 1 , wherein the biglycan polypeptide comprises amino acids 38-368 of SEQ ID NO: 9.
10. The method of claim 1 , wherein the biglycan polypeptide consists of amino acids 38-368 of SEQ ID NO: 9.