Ex vivo animal or challenge model as method to measure protective immunity directed against parasites and vaccines shown to be protective in the method
View Patent ↗The invention relates to an ex vivo animal or challenge model as a method to identify protective (recombinant) proteins and rapidly measure protective immunity in intestinal segments directed against parasites and vaccines directed against parasitic infections. The invention further relates to vaccines directed against infection with parasites, such as Fasciola hepatica , which vaccines contain protective (recombinant) proteins identified and shown to be protective in studies using the ex vivo model. The invention further relates to protective (recombinant) proteins obtained from newly excysted juveniles (NEJ) of Fasciola hepatica . The protective (recombinant) protein corresponding to an NEJ protein has an apparent molecular weight of 32 kD and an N-terminal amino acid sequence comprising the sequence XXDVSWPFWDRMYNY (SEQ ID NO:1).
1. An isolated nucleic acid molecule comprising a nucleotide molecule, or a nucleotide molecule complementary thereto, encoding a protein, said protein comprising an amino acid sequence of a proregion of a cathepsin-like protease that is specific for a newly excysted juvenile (NEJ) stage of Fasicola hepatica , wherein the protein comprises an amino acid molecule selected from the group consisting of SEQ ID NO:1, SEQ ID NO:24, and SEQ ID NO:26.
2. A host cell or expression system or vector comprising the isolated nucleic acid molecule of claim 1 .
3. The isolated nucleic acid molecule of claim 1 , wherein the protein is shown to be protective in a method comprising using an ex vivo animal or challenge model to measure protective immunity directed against a parasite.