Hepatitis C virus antibodies
This invention relates to humanized antibodies and fragments thereof which bind to hepatitis C virus E2 protein and methods of their use.
1. A humanized antibody that binds hepatitis C virus E2 protein or an antigen binding fragment thereof, wherein the humanized antibody or antigen binding fragment thereof comprises a heavy chain variable domain selected from the group consisting of SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, and SEQ ID NO:18.
2. A humanized antibody that binds hepatitis C virus E2 protein or an antigen binding fragment thereof, wherein the humanized antibody or the antigen binding fragment thereof comprises a light chain variable domain selected from the group consisting of SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:19, and SEQ ID NO:20.
3. The humanized antibody or antigen binding fragment thereof of claim 1 , wherein the humanized antibody or the antigen binding fragment thereof comprises a light chain variable domain selected from the group consisting of SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:19, and SEQ ID NO:20.
4. The humanized antibody or antigen binding fragment thereof of claim 3 , wherein the antigen binding fragment thereof is selected from the group consisting of a Fab fragment, a Fab′ fragment, a F(ab′)2 fragment, a scFv, a Fv, and a diabody.
5. A composition comprising the humanized antibody or antigen binding fragment thereof of claim 3 and a carrier or excipient.
6. A kit comprising the humanized antibody or antigen binding fragment thereof of claim 3 and instructions for administering said humanized antibody or antigen binding fragment thereof.
7. An in vitro assay method for identifying an agent that improves or enhances the efficacy of the neutralizing activity of the humanized antibody or antigen binding fragment thereof of claim 3 against hepatitis C virus, comprising the steps of (a) contacting said humanized antibody or antigen binding fragment thereof with an agent to be tested; and (b) determining whether the agent improves or enhances the efficacy of the humanized antibody or antigen binding fragment thereof in neutralizing the infectivity of hepatitis C virus.
8. A method for determining the presence or absence of hepatitis C virus in a sample from a subject, the method comprising the step of: contacting the sample with the humanized antibody or antigen binding fragment thereof of claim 3 .