Gene upregulated in cancers of the prostate
The present invention relates to a novel protein designated 20P2H8 which shares homology with several heterogeneous nuclear ribonucleoproteins (hnRNPs). A full length approximately 3600 bp 20P2H8 cDNA (SEQ ID NO: 1, encoding a 517 amino acid open reading frame (SEQ ID NO: 2), is provided herein.
1. An isolated nucleotide sequence comprising an uninterrupted sequence of nucleotides that encodes the polypeptide of SEQ ID NO:2.
2. The isolated nucleotide sequence of claim 1 , wherein the sequence comprises the nucleotide sequence of SEQ ID NO:1.
3. The isolated nucleotide sequence of claim 1 , wherein the sequence comprises nucleotides 451 to 2001 of SEQ ID NO:1.
4. An isolated recombinant expression vector comprising a nucleotide sequence that encodes the amino acid sequence of SEQ ID NO:2.
5. The recombinant expression vector of claim 4 , wherein the nucleotide sequence comprises the nucleotide sequence of SEQ ID NO:1.
6. The recombinant expression vector of claim 4 , wherein the nucleotide sequence comprises nucleotides 451 to 2001 of SEQ ID NO:1.
7. The recombinant vector of claim 4 , wherein the vector further comprises one or more regulatory sequences.
8. The recombinant vector of claim 4 , wherein the vector further comprises a stop codon associated with the nucleotide sequence.
9. The recombinant expression vector of claim 4 , wherein the vector is a viral vector.
10. The recombinant expression vector of claim 7 , wherein the viral vector is a vaccinia, fowlpox, canarypox, adenovirus, influenza, poliovirus, adeno-associated virus, lentivirus, or Sindbis virus.
11. An isolated host cell comprising a recombinant comprising a nucleotide sequence that encodes the amino acid sequence of SEQ ID NO:2.
12. The host cell of claim 11 , wherein the nucleotide sequence comprises the nucleotide sequence of SEQ ID NO:1.
13. The host cell of claim 11 , wherein the nucleotide sequence comprises nucleotides 451 to 2001 of SEQ ID NO:1.
14. A method of producing a polypeptide, comprising:
providing a host cell, wherein the host cell comprises an expression vector and wherein the expression vector comprises a nucleotide sequence encoding the polypeptide of SEQ ID NO:2; and
culturing the host cell under conditions to produce the polypeptide.
15. The method of claim 14 , wherein the host cell is prokaryotic or eukaryotic.
16. The method of claim 14 , wherein the nucleotide sequence encoding the polypeptide comprises the nucleotide sequence of SEQ ID NO: 1.
17. The method of claim 14 , further comprising purifying the polypeptide produced.