Production of fatty alcohols with fatty alcohol forming acyl-CoA reductases (FAR)
The disclosure relates to methods of producing fatty alcohols from recombinant host cells comprising genes encoding heterologous fatty acyl-CoA reductase (FAR) enzymes. The disclosure further relates to FAR enzymes and functional fragments thereof derived from marine bacterium and particularly marine gamma proteobacterium such as Marinobacter and Oceanobacter ; polynucleotides encoding the FAR enzymes and vectors and host cells comprising the same.
1. A method of producing a fatty alcohol-containing composition, the method comprising:
culturing recombinant Escherichia coli ( E. coli ) cells comprising a gene encoding a heterologous fatty acyl reductase (FAR) enzyme, wherein said heterologous FAR enzyme has a sequence with at least 90% amino acid sequence identity to SEQ ID NO:2, under conditions in which the FAR enzyme is expressed by said cells,
wherein said expression results in the secretion from the cells of fatty alcohols into a cell culture medium, thereby resulting in a fatty alcohol-containing composition.
2. The method of claim 1 , wherein the FAR enzyme has at least 95% amino acid sequence identity to SEQ ID NO:2.
3. The method of claim 1 , wherein the gene encoding the FAR enzyme comprises a nucleic acid sequence codon-optimized for expression in E. coli.
4. The method of claim 3 , wherein the gene encoding the FAR enzyme has the sequence of SEQ ID NO:1.
5. The method of claim 1 , wherein the gene encoding the FAR enzyme is integrated into the chromosome of the recombinant E. coli cells.
6. The method of claim 1 , wherein the gene encoding the FAR enzyme is contained in an expression vector in said E. coli cells.
7. The method of claim 1 , further comprising a step of isolating fatty alcohols from the cell culture medium.
8. The method of claim 1 , wherein the fatty alcohol-containing composition comprises at least 80% by weight of C14-C18 fatty alcohols.
9. The method of claim 1 , wherein at least 50% of the fatty alcohols produced by the E. coli cells are secreted into the culture medium.
10. The method according to claim 1 , wherein the fatty alcohol-containing composition comprises at least 0.5 g/L of fatty alcohol.
11. The method according to claim 10 , wherein the fatty alcohol-containing composition comprises at least 5.0 g/L of fatty alcohol.
12. The method of claim 1 , wherein the cells are cultured at a temperature in the range of 20° C. to 45° C.; a pH in the range of pH 5 to pH 7, and for a time in the range of from 16 hours to 144 hours.
13. The method of claim 12 , wherein the cells are cultured in the presence of fermentable sugars obtained from a cellulosic feedstock.
14. The method of claim 7 , wherein
a) the FAR enzyme has at least 95% amino acid sequence identity to SEQ ID NO:2;
b) at least 50% of the fatty alcohols produced by the E. coli cells are secreted into the culture medium;
c) the fatty alcohol-containing composition comprises at least 80% by weight of C14-C18 fatty alcohols, and
d) the fatty alcohol-containing composition comprises at least 5.0 g/L of fatty alcohol.