Method for making biochips
A method for immobilizing biological polymers such as DNA or proteins, on a solid support, by ionocovalent bond, for making biochips, and the resulting chips obtained by the method. Also, a kit for the preparation of the chips.
1. A product of the biochip type, comprising:
a flat solid support having a surface covered with zirconium phosphonate capable of coordination bonding with phosphate groups, and
wherein at least one phosphorylated protein carrying free phosphate groups OP(O)(OH) 2 is selectively immobilized on said surface by selective ionocovalent bonding between the free phosphate groups of the phosphorylated protein and the zirconium phosphonate.
2. The product according to claim 1 , wherein the zirconium phosphonate is bound to the surface of the support by way of a spacer molecule.
3. The product according to claim 2 , wherein the spacer molecule comprises a fatty acid chain carrying a phosphonate group to which the zirconium binds by selective ionocovalent bonding.
4. The product according to claim 2 , wherein the spacer molecule is octadecylphosphonic acid.
5. The product according to claim 1 , wherein the support is glass.
6. A method for making a product of the biochip type, as defined in claim 1 , comprising the selective immobilization of at least one phosphorylated protein carrying free phosphate groups on a solid support having a surface covered with zirconium phosphonate capable of coordination bonding with phosphate groups, and wherein the phosphorylated protein is selectively immobilized on said surface by selective ionocovalent bonding between the free phosphate groups of phosphorylated protein and the zirconium phosphonate.
7. The method according to claim 6 , further comprising a step of obtaining the phosphorylated protein carrying phosphate groups.
8. A kit for the preparation of a product of the biochip type as defined in claim 1 , comprising:
a solid support having a surface covered with zirconium phosphonate capable of coordination bonding with phosphate groups;
at least one phosphorylated protein carrying phosphate groups;
and,
optionally, reagents.
9. The kit according to claim 8 , wherein zirconium phosphonate is bound to the surface of the support by way of a spacer molecule.
10. The kit according to claim 9 , wherein the spacer molecule comprises a fatty acid chain carrying a phosphonate group to which zirconium binds by selective ionocovalent bonding.
11. The kit according to claim 9 , wherein the spacer molecule is octadecylphosphonic acid.
12. The kit according to claim 8 , wherein the support is glass.
13. The product according to claim 1 , wherein the phosphate groups act as anchoring functions.