Flow cell device
A microarray system is disclosed. The microarray system includes a microarray formed on a planar substrate and an incubation chamber formed around the microarray. The incubation chamber has a plurality of interior surfaces including a bottom surface on which the microarray is formed and a top surface that faces the bottom surface and is generally parallel to the bottom surface. At least one of a plurality of interior surfaces is a hydrophilic surface.
1. A device for detecting a target molecule, comprising:
a reaction chamber comprising
an inlet;
an outlet;
a target capturing surface that binds specifically to the target molecule,
a bottom surface of the reaction chamber, wherein the bottom surface comprises the target capturing surface, wherein the target capturing surface comprises a microarray; and
a top surface of the reaction chamber, wherein the top surface is a hydrophilic surface;
side walls of the reaction chamber, wherein the side walls are hydrophobic,
a waste chamber having an absorbent to allow liquids to be advanced by capillary action through the reaction chamber for washing; and
a channel of variable width connecting the reaction chamber to the waste chamber, wherein the channel comprises two sections, a first section at the end proximate to the reaction chamber and a second section at the end proximate to the waste chamber, wherein the first section has a diameter or cross-section area that is larger than the diameter or cross-section area of the second section,
wherein the second section further comprises two subsections: a first subsection next to the first section and a second subsection next to the waste chamber, wherein the first subsection forms an angle with the second subsection and has a diameter that is larger than the diameter of the second subsection.
2. The device of claim 1 , wherein the waste chamber comprises a vent.
3. The device of claim 1 , wherein the inlet port comprises a pierceable membrane or tape or a dome valve to allow washing to occur without allowing the content inside the reaction chamber to be liberated from the microarray system.