Method for manufacturing substrate for biochip
A substrate for biochips is manufactured so that the substrate has a substrate surface having a reaction region capable of reacting with biological substances and a non-reaction region not reacting with the biological substances, sunken bottomed wells formed in the substrate surface, and a layer of a material capable of reacting with the biological substances having a surface exposed only at the bottoms of the bottomed wells, the exposed surface forming the reaction region.
1. A method for manufacturing a substrate for a biochip comprising:
etching portions of a second layer disposed on a first layer and exhibiting the first layer; and
dipping the first layer and the second layer into a sodium hydrate solution to introduce a plurality of hydroxyl groups on the first layer and to remove a native oxide film on the second layer.
2. The method of claim 1 , further comprising etching portions of the second layer to form a plurality of grooves.
3. The method of claim 1 , further comprising linking each of a plurality of probe biomolecules to each of the hydroxyl groups.
4. The method of claim 3 , wherein the linking each of the probe biomolecules to each of the hydroxyl groups further comprises bonding a silane coupling agent to each of the hydroxyl groups.