Method for producing useful substance from cellulose-containing material
The present invention provides a method for producing a useful substance efficiently from cellulose without using any cellulase preparation. According to the disclosures of the present specification, in the production of a useful substance from a cellulose-containing material, the cellulose-containing material is brought into contact with an ionic liquid to cause the ionic liquid to permeate the cellulose-containing material, and a carbon source comprising the cellulose in the cellulose-containing material is simultaneously saccharified and fermented by a cellulase-producing microorganism in the presence of the ionic liquid.
1. A method for producing a useful substance from a cellulose-containing material, comprising:
bringing a cellulose-containing material into contact with an ionic liquid to cause the ionic liquid to permeate the cellulose-containing material; and
simultaneously saccharifying and fermenting a carbon source in the presence of the ionic liquid that comprises the cellulose in the cellulose-containing material using a cellulase-producing microorganism to produce the useful substance, wherein a concentration of the ionic liquid ranges from 50 to 500 mM, and wherein the useful substance produced is selected from the group consisting of alcohols, organic acids, glycerin, and plastics.
2. The method according to claim 1 , wherein the bringing of the cellulose-containing material into contact with the ionic liquid comprises heating the cellulose-containing material and the ionic liquid.
3. The method according to claim 1 , further comprising:
performing solid-liquid separation for separating a cellulose-containing fraction from a non-cellulose-containing fraction after bringing the cellulose-containing material into contact with the ionic liquid.
4. The method according to claim 1 , wherein the ionic liquid comprises a hydrophilic ionic liquid.
5. The method according to claim 1 , wherein the microorganism is a recombinant yeast expressing cellulase.
6. The method according to claim 5 , wherein the recombinant yeast is a yeast that secretes or surface displays the cellulase.
7. The method according to claim 3 , further comprising:
collecting the ionic liquid that is in the non-cellulose-containing fraction that has been separated in the solid-liquid separation, and supplying the ionic liquid to the cellulose-containing material to bring the cellulose-containing material into contact with the ionic liquid.
8. The method according to claim 1 , wherein the microorganism is a yeast secreting or surface displaying cellulase.
9. The method according to claim 1 , wherein the cellulose-containing material is a material containing polymers and derivatives of polymers formed from glucose by polymerization with β-1,4-glucoside bonds.
10. The method according to claim 1 , wherein the cellulase-producing microorganism is selected from the group consisting of Clostridium, Trichoderma, Aspergillus, Escherichia coli, Bacillus subtilis , yeasts, genetically modified yeasts, and combinations thereof,
wherein the genetically modified yeast is modified to produce cellulase.
11. The method according to claim 1 , wherein the substance produced is selected from ethanol, propanol, isopropanol, butanol, and isobutanol.
12. The method according to claim 1 , wherein the concentration of the ionic liquid ranges from 50 to 300 mM.
13. The method according to claim 1 , wherein the concentration of the ionic liquid ranges from 50 to 200 mM.
14. The method according to claim 1 , wherein the cellulose-containing material comprises lignan.
15. The method according to claim 1 , wherein the ionic liquid has 1-ethyl-3-methyl-imdazolium as a cationic species.
16. A method for producing a useful substance from a cellulose-containing material, comprising:
bringing a cellulose-containing material into contact with an ionic liquid to cause the ionic liquid to permeate the cellulose-containing material; and
simultaneously saccharifying and fermenting a carbon source in the presence of the ionic liquid that comprises the cellulose in the cellulose-containing material using a cellulase-producing microorganism to produce the useful substance,
wherein a concentration of the ionic liquid ranges from 50 to 500 mM, wherein the useful substance produced is selected from the group consisting of alcohols, organic acids, glycerin, and plastics, and
wherein the microorganism comprises a yeast secreting or yeast surface displaying cellulase.
17. The method according to claim 16 , wherein the microorganism is a yeast surface displaying cellulase.