ULTRA DARK FIELD MICROSCOPE
A fluorescence microscope includes a nearly monochromatic light source, a Brewster angle wedge, and an optical system for irradiating a sample with a light beam from the light source and directing fluorescence light from said sample onto the Brewster angle wedge. Collection optics are provided for focusing a hyper-spectral, wide angle and dark field image of the sample from the Brewster angle wedge onto recording optics.
1 . A fluorescence microscope comprising:
a nearly monochromatic light source;
a Brewster angle wedge;
an optical system for irradiating a sample with a light beam from the light source and directing fluorescence light from said sample onto the Brewster angle wedge; and
collection optics for focusing a hyper-spectral, wide angle and dark field image of the sample from the Brewster angle wedge onto recording optics.
2 . The fluorescence microscope according to claim 1 wherein said optical system comprises a filter/beam splitter for blocking off band light from the light source and directing the fluorescence light onto the Brewster angle wedge.
3 . The fluorescence microscope according to claim 2 wherein said optical system further comprises magnification optics for magnification of said sample.
4 . The fluorescence microscope according to claim 3 wherein said optical system further comprises a collimator optic for rendering parallel fluorescence light onto the Brewster angle wedge.
5 . The fluorescence microscope according to claim 1 wherein said optical system is configured for establishing confocal focus between the sample and recoding optics.
6 . The fluorescence microscope according to claim 5 wherein said optical system further comprises a beam splitter for directing fluorescent light onto a monitor.
7 . The fluorescence microscope according to claim 6 wherein said optical system further comprises confocal apparatus to prevent out-of-focus fluorescence from reaching the recording optics.
8 . The fluorescence microscope according to claim 7 wherein said optical system further comprises magnification optics for magnification of said sample.
9 . The fluorescence microscope according to claim 8 wherein said optical system further comprises a collimation optic for rendering parallel the fluorescence light onto the Brewster angle wedge.
10 . A fluorescence microscopy method comprising:
providing a Brewster angle wedge;
irradiating a sample with a nearly monochromatic light source for producing a fluorescent image of the sample, and a molarity map of specific chemicals or molecules;
directing the fluorescent light onto the Brewster angle wedge to produce a hyper-spectral, wide angle, and dark field image of the sample; and
directing the fluorescent light of the sample onto recording optics.
11 . The method according to claim 10 further utilizing a filter/beam splitter to block off based light from the light source and direct the fluorescence light onto the Brewster angle wedge.
12 . The method according to claim 11 further comprising magnification of said sample.
13 . The method according to claim 12 further comprising providing a collimator optic for rendering parallel fluorescence light onto the Brewster angle wedge.
14 . The method according to claim 5 , further establishing confocal focus between the sample and recoding optics.
15 . The method according to claim 10 , further providing a beam splitter for directing fluorescence light onto a monitor.
16 . The method according to claim 5 , further comprising providing confocal apparatus to prevent out-of-focus fluorescence from reaching the recording optics.
17 . The method according to claim 5 , further comprising magnification of said sample.
18 . The method according to claim 17 , further comprising providing a collimation optic for rendering parallel the fluorescence light onto the Brewster angle wedge.