Polynucleotides encoding for fusion proteins with natriuresis activity
View Patent ↗This document provides methods and material related to natriuretic polypeptides. For example, substantially pure polypeptides having a natriuretic peptide activity, nucleic acids encoding polypeptides having a natriuretic peptide activity, host cells containing such nucleic acids, and methods for inducing a natriuretic or diuretic activity within a mammal are provided.
1. An isolated nucleic acid comprising a nucleotide sequence encoding a polypeptide that comprises a first amino acid sequence and a second amino acid sequence, wherein said first amino acid sequence comprises the sequence of a natriuretic polypeptide having natriuresis activity, wherein said second amino acid sequence comprises the amino acid sequence as set forth in SEQ ID NO:1 or the amino acid sequence as set forth in SEQ ID NO: 1 having less than five amino acid additions, subtractions, and substitutions, and wherein said polypeptide has said natriuresis activity.
2. The nucleic acid of claim 1 , wherein said first amino acid sequence comprises an amino acid sequence present in an ANP polypeptide.
3. The nucleic acid of claim 1 , wherein said first amino acid sequence comprises an amino acid sequence present in a BNP polypeptide.
4. The nucleic acid of claim 1 , wherein said first amino acid sequence comprises an amino acid sequence present in a CNP polypeptide.
5. The nucleic acid of claim 1 , wherein said first amino acid sequence comprises an amino acid sequence present in a DNP polypeptide.
6. The nucleic acid of claim 1 , wherein said first amino acid sequence comprises an amino acid sequence present in a salmon natriuretic factor polypeptide.
7. The nucleic acid of claim 1 , wherein said second amino acid sequence comprises the amino acid sequence as set forth in SEQ ID NO:1.
8. The nucleic acid of claim 1 , wherein said second amino acid sequence comprises the amino acid sequence as set forth in SEQ ID NO:1 having less than five amino acid additions, subtractions, and substitutions.
9. The nucleic acid of claim 1 , wherein said second amino acid sequence comprises the amino acid sequence as set forth in SEQ ID NO:1 having less than four amino acid additions, subtractions, and substitutions.
10. A host cell comprising an exogenous nucleic acid comprising a nucleotide sequence encoding a polypeptide that comprises a first amino acid sequence and a second amino acid sequence, wherein said first amino acid sequence comprises the sequence of a natriuretic polypeptide having natriuresis activity, wherein said second amino acid sequence comprises the amino acid sequence as set forth in SEQ ID NO:1 or the amino acid sequence as set forth in SEQ ID NO: 1 having less than five conservative amino acid additions, subtractions, and substitutions, and wherein said polypeptide has said natriuresis activity.
11. The nucleic acid of claim 1 , wherein said nucleic acid encodes an amino acid sequence as set forth in SEQ ID NO:2.