Astrovirus species
Provided herein are sequences of the genomes and encoded proteins of new astrovirus species, and variants thereof. Also provided are methods of detecting the new astrovirus species and diagnosing astrovirus infection, methods of treating or preventing astrovirus infection, and methods for identifying anti-astrovirus compounds. Provided are two new astrovirus species named HMOAstV-A and HMOAstV-B, and both are distantly related to known astroviruses. Also provided is a new method of classifying astroviruses, where there are three groups of human astroviruses, including HAstV, AstV-MLB, and HMOAstV.
1. A recombinant vector comprising an operative promoter and a heterologous nucleic acid molecule comprising:
a) A sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:5, or the full-length complement thereof,
b) A sequence comprising at least 50, 100, 150 contiguous nucleotides of SEQ ID NO:1, SEQ ID NO:5, or the full-length complement thereof, or
c) A sequence having at least 75% identity to SEQ ID NO:1, SEQ ID NO:5, or a full-length complement thereof.
2. The recombinant vector of claim 1 , wherein the nucleic acid molecule comprises at least 50, 100 or 150 contiguous nucleotides of SEQ ID NO:1, SEQ ID NO: 5 , or the full-length complement thereof.
3. The recombinant vector of claim 1 , wherein the sequence is at least 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical to SEQ ID NO:1, SEQ ID NO:5, or the full-length complement thereof.
4. An isolated host cell comprising the recombinant vector of claim 1 .
5. A method of detecting an astrovirus nucleic acid comprising:
(a) contacting a sample suspected of containing an astrovirus nucleic acid with a nucleic acid molecule comprising at least 50, 100 or 150 contiguous nucleotides of SEQ ID NO:1, SEQ ID NO:5, or the full-length complement thereof; and (b) detecting the presence or absence of hybridization.
6. A method of detecting an astrovirus nucleic acid comprising:
(a) amplifying the nucleic acid of a sample suspected of containing an astrovirus nucleic acid with at least one nucleic acid molecule comprising at least 50, 100 or 150 contiguous nucleotides of SEQ ID NO:1, SEQ ID NO:5, or the full-length complement thereof, to produce an amplification product; and
(b) detecting the presence of an amplification product, thereby detecting the presence of the astrovirus nucleic acid.
7. A kit for detecting an astrovirus nucleic acid comprising a detectable label attached to an isolated nucleic acid molecule comprising at least 50, 100 or 150 contiguous nucleotides of SEQ ID NO:1, SEQ ID NO:5, or the full-length complement thereof.
8. A method of assaying for an anti-astrovirus compound comprising:
(a) contacting a sample containing an astrovirus comprising a nucleotide sequence having at least 75% identity to SEQ ID NO:1, SEQ ID NO:5, or a full-length complement thereof, with a test compound; and
(b) determining whether the test compound inhibits astrovirus replication, wherein inhibition of astrovirus replication indicates that the test compound is an anti-astrovirus compound.