Immunoassay assembly and methods of use
The present invention relates to an improved system for efficiently and accurately performing immunoassays, such as ELISAs. The invention provides an immunoassay assembly which includes a flow-through unit and an aspiration pump. The immunoassay flow-through unit includes an outer seal; at least one bed support; an inner seal; and a packed non-porous bed. The unit is releasably attached to an aspiration pump which enables the controlled flow rate of liquid passing through the packed bed of the flow-through unit. The invention also provides a method of using the immunoassay assembly to identify analytical targets of interest.
1. An immunoassay or selective adsorption assembly comprising a flow-through assay unit and an aspiration pump; wherein the flow-through assay unit includes a pair of porous bed supports trapping between them solid phase support beads with a reaction reagent mounted on their surface, an open proximal end, and an open distal end of smaller diameter than the proximal end; wherein the aspiration pump includes a pump block, a piston, and an inlet port formed in the pump block and sized to receive the distal end; the aspiration pump is in fluid communication with the flow-through assay unit via the distal end to draw fluid therethrough; the aspiration pump is vertically oriented with the inlet port being conical at a top of the aspiration pump; and the assay unit has a corresponding tapered opening at the distal end so that the assay unit is mountable upon the pump in fluid-tight fashion.
2. An immunoassay or selective adsorption assembly as claimed in claim 1 wherein the flow-through assay unit also includes a plurality of sealing surfaces of frusto-conical shape and of differing sizes to accommodate fluid tight communication with a variety of fluid handling equipment.