Anti-inflammatory macrophages and uses thereof
The invention relates to a purified, novel anti-inflammatory population of macrophage and methods of making and using such macrophage.
1. An ex vivo generated population of human anti-inflammatory macrophages that is IL-6 high, IL-10 high, IL-12 low, and TNF-α low.
2. The population of human anti-inflammatory macrophages of claim 1 , wherein the macrophages express CD 206.
3. A composition comprising:
human MSC-educated macrophages effective to alleviate a condition associated with inflammation; and
a pharmaceutically acceptable carrier.
4. The composition of claim 3 , wherein the carrier is selected from the group consisting of liquid, oil, lotion, salve, cream, foam, gel, paste, powder, film, and hydrogel.
5. The composition of claim 3 , wherein the human MSC-educated macrophages are characterized by a IL-6 high, IL-10 high, IL-12 low, and TNF-α low immunophenotype.
6. The composition of claim 3 , wherein the condition is a non-healing wound.
7. The composition of claim 3 , wherein the condition is diabetes.
8. The population of human anti-inflammatory macrophages of claim 1 , wherein the macrophages are obtained from peripheral blood.
9. The composition of claim 3 , wherein the human MSC-educated macrophages are derived from human peripheral blood macrophages.