Implant surface treatment method having tissues integrated
The present disclosure uses different kinds of surface treatment processes on titanium-made dental implants. The growth and attachment conditions of bone cells (MC3T3-E), fibroblasts (NIH 3T3) and epidermal cells (XB-2) on the metal surface of titanium slices with different surface treatments are observed. Tetra-calcium phosphate is used to perform secondary sand-blasting process to clean up the metal surface and provide calcium ions for osteoblastoma physiology. Thus, by adjusting the cells adhesive and proliferative abilities, the success rate of the clinical applications in dental implant is improved.
1. An implant surface treatment method to analyze the effects of surface treatment on cell growth, comprising the steps of:
(a) treating one portion of a surface of a titanium (Ti) metal of an implant through a sandblasted, large-grit, acid-etched (SLA) surface treatment to obtain roughness;
(b) treating a second portion of a surface of a titanium (Ti) metal of an said implant through a sandblasted, large grit, acid etched (SLA) surface treatment and using a calcium-rich phosphate salt to clean and be embedded into said Ti metal through secondary grit-blasting, wherein the calcium-rich phosphate salt provides calcium ions and phosphate ions;
(c) leaving a third portion of the surface of a said titanium metal implant untreated;
(d) culturing a bone cell, a fibroblast and an epidermal cell on the surface of the implant, wherein said bone cell is MC3T3-E1, said fibroblast is NIH 3T3 and said epidermal cell is XB-2, to identify the amount of sandblasting and cleaning to result in activation of growth of said epidermal cell and inhibition of growth of said fibroblast on the third portion of the implant surface, and activation of growth of said bone cell and inhibition of growth of said fibroblast on the second portion of the implant surface; and
(e) analyzing growth of each of the cell types on the treated implant.
2. The method according to claim 1 , wherein said method treats an object selected from a group consisting of an implant stem and an abutment.
3. The method according to claim 1 , wherein, on using a blasting sand not assimilated to human body in grit-blasting, said implant is cleaned by using a material having a smaller particle diameter than that of said blasting sand not assimilated to human body; and wherein said material is a calcium-rich phosphate salt having a particle diameter of less than 44 micrometers (μm).
4. The method according to claim 3 , wherein said calcium-rich phosphate salt has a calcium-to-phosphate atomic ratio larger than or to equal to the value of 1.5 and is selected from the group consisting of: Ca 4 (PO 4 ) 2 O, CaHPO 4 .2H 2 O, CaHPO 4 , Ca 8 H 2 (PO 4 ) 6 .5H 2 O, alpha-Ca 3 (PO 4 ) 2 , beta-Ca 3 (PO 4 ) 2 , Ca 2 P 2 O 7 , Ca 2 H 2 P 2 O 8 and an apatite.
5. The method according to claim 1 , wherein, upon implantation into the mouth, the smooth third portion of the implant surface extends 0.1-1.5 mm below the surface of the skin and contacts epidermal cells, and wherein the rough first portion of the implant surface extends below the smooth third portion of the implant surface to a depth of 2.9 mm and contacts fibroblasts, and wherein the rough first portion of the implant surface has a surface roughness of 0.2-6 microns.
6. The method of claim 1 , wherein the calcium-rich phosphate salt is tetracalcium phosphate (TTCP).
7. The method of claim 1 , wherein from top to bottom, the treatment results in the surface of the implant being smooth, then rough, and then rough with calcium ions and phosphate ions added by the calcium-rich phosphate salt, wherein the calcium-to-phosphate atomic ratio in the calcium-rich phosphate salt is larger than or equal to the value of 1.5.
8. The method of claim 1 , wherein the method for analyzing growth is by cell morphology, cell number and/or OD.