CELLULOSE PRODUCTION BY FACULTATIVELY ANAEROBIC MICROORGANISMS
A method for producing bacterial cellulose, said method comprising culturing a biologically pure culture of a cellulose-producing Proteus strain in a liquid medium suitable for culturing facultatively anaerobic microorganisms, separating bacterial cellulose produced in said liquid medium from said liquid medium, washing said separated bacterial cellulose and drying said bacterial cellulose. The cellulose-producing Proteus strain is preferably a Proteus myxofaciens strain, preferably strain IDAC 071005-01 or strain ATCC 19692. The liquid medium is provided with a carbohydrate substrate containing at least one sugar selected from the group consisting of glucose, sucrose, fructose, lactose, xylose, and rhamnose. A bacterial cellulose product produced by culturing a biologically pure culture of a cellulose-producing Proteus strain in a liquid medium suitable for culturing facultatively anaerobic microorganisms.
1 . A method for producing bacterial cellulose, comprising:
culturing a biologically pure culture of a cellulose-producing Proteus myxofaciens ATCC 19692 strain in a liquid medium suitable for culturing facultatively anaerobic microorganisms such that the strain produces bacterial cellulose;
recovering bacterial cellulose from the liquid medium;
washing the recovered bacterial cellulose with water; and
drying said bacterial cellulose.
2 . The method according to claim 1 wherein the liquid medium is suitable for culturing facultatively anaerobic microorganisms under aerobic conditions.
3 . The method according to claim 2 wherein the liquid medium is provided with a carbohydrate substrate containing therein at least one sugar selected from the group consisting of glucose, sucrose, fructose, lactose, xylose, and rhamnose.
4 . The method according to claim 2 wherein the liquid medium is provided with a pH selected from the range of 5 to 10.
5 . The method according to claim 2 wherein the liquid medium is provided with a pH selected from the range of 6 to 9.
6 . The method according to claim 2 wherein the liquid medium is provided with a pH selected from the range of 7 to 8.5.
7 . The method according to claim 2 wherein the liquid medium is provided with a buffer.
8 . The method according to claim 2 wherein the buffer is selected from the group consisting of sodium chloride and phosphate.
9 . The method according to claim 2 wherein the buffer comprises a mixture of sodium chloride and phosphate.
10 . The method according to claim 1 wherein the liquid medium is suitable for culturing facultatively anaerobic microorganisms under anaerobic conditions.
11 . The method according to claim 10 wherein the liquid medium is provided with a carbohydrate substrate containing therein at least one sugar selected from the group consisting of glucose, sucrose, fructose, lactose, xylose, and rhamnose.
12 . The method according to claim 10 wherein the liquid medium is provided with a pH selected from the range of 5 to 10.
13 . The method according to claim 10 wherein the liquid medium is provided with a pH selected from the range of 6 to 9.
14 . The method according to claim 10 wherein the liquid medium is provided with a pH selected from the range of 7 to 8.5.
15 . The method according to claim 10 wherein the liquid medium is provided with a buffer.
16 . The method according to claim 15 wherein the buffer is selected from the group consisting of sodium chloride and phosphate.
17 . The method according to claim 15 wherein the buffer comprises a mixture of sodium chloride and phosphate.
18 . A bacterial cellulose product produced according to the method of claim 2 .
19 . A bacterial cellulose product produced according to the method of claim 10 .