Magnetic lysis method and device
View Patent ↗A method for lysing cells is disclosed. The method includes stirring cells with a magnetic stir element in the presence of a plurality of cell lysis beads at a speed sufficient to lyse the cells. Also disclosed is a device for lysing cells. The device includes a container having a magnetic stir element and a plurality of cell lysis beads disposed therein. The container is dimensioned to allow rotation of the magnetic stir element inside the container.
1. A method for lysing cells, comprising:
stirring a liquid suspension containing one or more cells of interest with a magnetic stir element in a chamber in the presence of a plurality of beads, wherein said magnetic stir element drives motion of said plurality of beads and collides with said beads and wherein said magnetic stir element rotates at a speed for a period of time sufficient to lyse said one or more cells of interest.
2. The method of claim 1 , further comprising: suspending said one or more cells in a liquid medium and forming said liquid suspension.
3. The method of claim 1 , wherein said beads comprises a material selected from the group consisting of plastics, glass, ceramics, and metals.
4. The method of claim 3 , wherein said beads are silica beads.
5. The method of claim 1 , wherein said beads have diameters within the range of 10-1000 μm.
6. The method of claim 1 , wherein said magnetic stir element comprises a metal or an alloy.
7. The method of claim 1 , wherein said magnetic stir element has a shape selected from the group consisting of circular shape, square shape, rectangular shape, curved rod shape, cross-section rectangular shape, disc shape, rod shape, annulus shape, crescent shape, trapezoidal shape and two-pronged tuning fork shape.
8. The method of claim 1 , wherein said cells of interest are eukaryotic cells or prokaryotic cells and wherein said liquid suspension contains said cells at a concentration ranging from 1 to 1×10 10 cells/ml.
9. The method of claim 1 , wherein said magnetic stir element rotates at a speed of 1000-6000 rpm and said period of time is 90-120 seconds.
10. A method for lysing a cell, comprising:
applying a magnetic field to a chamber comprising a cell of interest, a stirrer element, and a plurality of beads, wherein said magnetic field causes said stirrer element to rotate at a speed for a period of time so that said stirrer element collides with said beads with enough force to cause disintegration of said cell.
11. The method of claim 10 , wherein said beads comprise glass beads, plastic beads, ceramic beads, metal beads or a mixture thereof.
12. The method of claim 10 , wherein said stirrer element comprises stainless steel.
13. The method of claim 10 , wherein said stirrer element comprises an alloy core coated with a polymer.
14. The method of claim 13 , wherein said alloy core comprises neodymium iron boron or samarium cobalt.
15. The method of claim 13 , wherein said polymer is a biocompatible polymer.
16. The method of claim 15 , wherein said biocompatible polymer is polytetrafluoroethylene (PTFE) or parylene.
17. A method for purifying nucleic acids from a cell, comprising:
applying a magnetic field to a vessel containing said cell, a stir element, and a plurality of beads, wherein said magnetic field causes said stir element to rotate at a speed for a period of time so that said stir element collides with said plurality of beads, thereby producing a cell lysate; and
isolating nucleic acids from said cell lysate.
18. The method of claim 17 , wherein said magnetic stir element rotates at a speed of 1000-6000 rpm and said period of time is 30 or 120 seconds.
19. A method for amplifying a polynucleotide from a cell, comprising:
applying a magnetic field to a vessel containing said cell, a stir element, and a plurality of beads, wherein said magnetic field causes said stir element to rotate at a speed for a period of time so that said stir element collides with said plurality of beads, thereby producing a cell lysate; and
amplifying said polynucleotide from said cell lysate.
20. The method of claim 19 , wherein said magnetic stir element rotates at a speed of 1000-6000 rpm and said period of time is 30 or 120 seconds.
21. A method for detecting a polynucleotide from a cell, comprising:
applying a magnetic field to a vessel containing said cell, a stir element, and a plurality of beads, wherein said magnetic field causes said stir element to rotate at a speed for a period of time so that said stir element collides with said plurality of beads, thereby producing a cell lysate; and
detecting said polynucleotide from said cell lysate.
22. The method of claim 21 , wherein said detecting step comprises:
isolating nucleic acids from said cell lysate and producing isolated nucleic acids;
amplifying said polynucleotide from said isolated nucleic acids and producing amplified polynucleotides; and
detecting said amplified polynucleotides.
23. The method of claim 21 , wherein said magnetic stir element rotates at a speed of 1000-6000 rpm and said period of time is 30 or 120 seconds.