Integrated cartridge
An integrated cartridge for sample processing and analysis is disclosed. The integrated cartridge contains a sample preparation chamber having a sample inlet and a sample outlet, and a sample purification chamber adapted to receive a replaceable sample purification unit containing a housing and an extraction filter inside the housing. The extraction filter specifically binds to a molecule of interest. The sample purification chamber has a sample inlet that is in fluid communication with the sample outlet of the sample preparation chamber. Also disclosed is a microarray-based sample analysis (MBSA) system.
1. An integrated cartridge for sample processing and analysis, comprising:
a sample purification chamber configured to receive a replaceable sample purification unit containing a housing and an extraction filter inside the housing, wherein the extraction filter is a silica filter that specifically binds to an analyte;
a sample analysis unit in fluid communication with the sample purification chamber, wherein the sample analysis unit comprises a microarray chamber comprising a microarray for analysis of analyte eluted from the silica filter; and
a plurality of pin valves that control liquid movement within the cartridge.
2. The integrated cartridge of claim 1 , further comprising a waste chamber.
3. The integrated cartridge of claim 1 , wherein the microarray chamber is configured to perform an amplification reaction within the chamber.
4. The integrated cartridge of claim 1 , wherein the one or more plurality of pin valves are controlled by one or more linear actuators.
5. The integrated cartridge of claim 1 , further comprising microfluidic channels that facilitate fluid movement within the integrated cartridge, wherein interior surfaces of the microchannels are fully or partially covered or coated with a hydrophilic film to reduce bubble trapping inside the microfluidic channels.
6. The integrated cartridge of claim 5 , wherein the microfluidic channels have diameters that are sufficiently large to reduce backpressure and enable reproducible injection molded features.
7. The integrated cartridge of claim 1 , wherein the microarray is a DNA array.
8. The integrated cartridge of claim 1 , wherein the microarray is a protein or peptide array.
9. The integrated cartridge of claim 1 , wherein the microarray is an antibody array.
10. The integrated cartridge of claim 1 , wherein the plurality of pin valves are electronically controlled.
11. The integrated cartridge of claim 10 , wherein the plurality of pin valves are controlled by a linear actuator panel.
12. An integrated cartridge for sample processing and analysis, comprising:
means configured to receive a replaceable sample purification unit containing a housing and an extraction filter inside the housing, wherein the extraction filter comprises a silica filter that specifically binds to nucleic acids;
a sample analysis unit in fluid communication with the sample purification chamber, wherein the sample analysis unit comprises a microarray chamber comprising a DNA microarray for analysis of nucleic acids eluted from the silica filter; and
a plurality of pin valves that control liquid movement within the cartridge.