Method facilitating removal of bioorganic stains from surfaces
Protein-polymer composite materials are provided according to embodiments of the present invention that include an admixture of a polymer resin, a surfactant and a non-aqueous organic solvent. An aqueous solution containing bioactive proteins is mixed with the admixture. The emulsion is mixed with a crosslinker to produce a curable composition. The curable composition is cured, thereby producing the protein-polymer composite material that is useful for facilitating removal of bioorganic stains.
1. A method for facilitating removal of a bioorganic stain from a surface comprising:
providing a curable polymeric coating comprising a polymer resin, a surfactant, a non-aqueous organic solvent having a log P in the range of −0.5-2, inclusive, where log P is the base ten logarithm of the ratio of solubility of the non-aqueous organic solvent in n-octanol to solubility of the non-aqueous organic solvent in water, and a cross-linker;
dispersing an amylase or analogue thereof having amylase activity within said curable polymeric coating;
curing said curable polymeric coating such that said amylase or analogue thereof having amylase activity is capable of enzymatically cleaving a component of said bioorganic stain when contacting said curable polymeric coating; and
contacting said curable polymeric coating with said bioorganic stain.
2. The method of claim 1 , wherein the polymer resin is a hydroxyl-functionalized acrylate resin.
3. The method of claim 2 , wherein the crosslinker is a polyisocyanate.
4. The method of claim 1 , wherein said amylase or analog therof having amylase activity is an α-amylase.
5. The method of claim 1 , further comprising addition of one or more additives to said curable polymeric coating.
6. The method of claim 1 wherein said amylase or analogue thereof having amylase activity is in the form of particles in said curable polymeric coating, the average particle size of said particles in the curable polymeric coating is in the range of 1 nm to 10 μm average diameter, inclusive.
7. The method of claim 1 wherein a surface activity of said cured polymeric coating is at least 0.1 Unit/cm 2 wherein said surface activity is amylase units present in a square centimeter of surface area.
8. The method of claim 1 wherein said bioorganic stain comprises starch.
9. The method of claim 1 wherein said bioorganic stain comprises food.
10. The method of claim 1 wherein said food comprises light mayonnaise, barbeque sauce, tartar sauce, potato, fruit, or combinations thereof.
11. The method of claim 1 , further comprising applying the curable polymeric coating to a substrate prior to curing, and subsequent to said step of dispersing.
12. The method of claim 11 wherein said curing comprises thermal curing.
13. The method of claim 11 , wherein said curing comprises curing using actinic radiation.