Polypeptides having xylanase activity and polynucleotides encoding same
View Patent ↗The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
1. An isolated polypeptide having xylanase activity, selected from the group consisting of:
(a) a polypeptide comprising an amino acid sequence having at least 98% identity to amino acids 20 to 407 of SEQ ID NO: 2;
(b) a polypeptide encoded by a polynucleotide comprising nucleotides 58 to 1439 of SEQ ID NO: 1; and
(c) a fragment of SEQ ID NO: 2 having xylanase activity.
2. The polypeptide of claim 1 , comprising or consisting of the amino acid sequence of SEQ ID NO: 2 or amino acids 20 to 407 of SEQ ID NO: 2; or a fragment thereof having xylanase activity.
3. The polypeptide of claim 1 , which is encoded by the polynucleotide contained in plasmid pGEM-T-Ppin3 which is contained in E. coli DSM 22922.
4. The polypeptide of claim 1 , comprising an amino acid sequence having at least 99% identity to amino acids 20 to 407 of SEQ ID NO: 2.
5. A method of producing the polypeptide of claim 1 , comprising: (a) cultivating a cell, which in its wild-type form produces the polypeptide, under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide.
6. A method for degrading or converting a cellulosic material or a xylan-containing material, comprising: treating the cellulosic material or the xylan-containing material with an enzyme composition in the presence of the polypeptide having xylanase activity of claim 1 .
7. The method of claim 6 , further comprising recovering the degraded cellulosic material or the degraded xylan-containing material.
8. The method of claim 6 , wherein the cellulosic material or the xylan-containing material is pretreated.
9. The method of claim 7 , wherein the degraded cellulosic material or the degraded xylan-containing material is a sugar.
10. A method for producing a fermentation product, comprising:
(a) saccharifying a cellulosic material or a xylan-containing material with an enzyme composition in the presence of the polypeptide having xylanase activity of claim 1 ;
(b) fermenting the saccharified cellulosic material or the saccharified xylan-containing material with one or more fermenting microorganisms to produce the fermentation product; and
(c) recovering the fermentation product from the fermentation.
11. The method of claim 10 , wherein the cellulosic material or the xylan-containing material is pretreated.
12. The method of claim 10 , wherein steps (a) and (b) are performed simultaneously in a simultaneous saccharification and fermentation.
13. A method of fermenting a cellulosic material or a xylan-containing material, comprising: fermenting the cellulosic material or the xylan-containing material with one or more fermenting microorganisms, wherein the cellulosic material or the xylan-containing material is saccharified with an enzyme composition in the presence of a polypeptide having xylanase activity of claim 1 .
14. The method of claim 13 , wherein the fermenting of the cellulosic material or the xylan-containing material produces a fermentation product.
15. The method of claim 13 , wherein the cellulosic material or the xylan-containing material is pretreated before saccharification.