Biosensor membranes composed of polymers containing heterocyclic nitrogens
Novel membranes comprising various polymers containing heterocyclic nitrogen groups are described. These membranes are usefully employed in electrochemical sensors, such as amperometric biosensors. More particularly, these membranes effectively regulate a flux of analyte to a measurement electrode in an electrochemical sensor, thereby improving the functioning of the electrochemical sensor over a significant range of analyte concentrations. Electrochemical sensors equipped with such membranes are also described.
1. A polymer complex comprising:
a first polymer comprising an analyte responsive enzyme and a mediator, wherein the mediator is chemically bonded to the first polymer; and
a second polymer disposed on top and in contact with the first polymer, the second polymer comprising a polymer having the formula:
wherein the solid horizontal line represents a polymer backbone;
A is an alkyl substituted with a water-soluble constituent;
D is selected from phenylalkyl, alkoxystyrene, hydroxyalkyl, alkoxyalkyl, alkoxycarbonylalkyl, a poly(ethylene glycol)-containing constituent, and a polyhydroxyl-containing constituent; and
each of n, 1, p and q is independently a positive number.
2. The polymer complex of claim 1 , wherein the mediator is covalently bonded to the first polymer.
3. The polymer complex of claim 2 , wherein the mediator forms one or more of carbon-carbon or carbon-nitrogen covalent bonds with the first polymer.
4. The polymer complex of claim 2 , wherein the mediator is covalently bonded to the first polymer through a linker moiety.
5. The polymer complex of claim 2 , wherein the mediator is covalently bonded to the first polymer through one or more ligands of the mediator.
6. The polymer complex of claim 5 , wherein the one or more ligands comprises a substituted or unsubstituted heterocyclic nitrogen-containing moiety.
7. The polymer complex of claim 1 , wherein the first polymer is crosslinked.
8. The polymer complex of claim 7 , wherein the mediator is crosslinked with the first polymer.
9. The polymer complex of claim 8 , the mediator is crosslinked with the first polymer through one or more crosslinking agents.
10. The polymer complex of claim 9 , wherein the one or more crosslinking agents is an unsubstituted epoxide.
11. The polymer complex of claim 1 , wherein the analyte-responsive enzyme is a glucose-responsive enzyme.
12. The polymer complex of claim 11 , wherein the analyte-responsive enzyme is glucose oxidase (GOx).
13. The polymer complex of claim 1 , wherein the analyte-responsive enzyme further comprises an enzyme cofactor.
14. The polymer complex of claim 13 , wherein the enzyme cofactor is flavin adenine dinucleotide.
15. The polymer complex of claim 1 , wherein the mediator comprises ruthenium or osmium.
16. The polymer complex of claim 1 , wherein A is negatively charged.
17. The polymer complex of claim 1 , wherein A is selected from a sulfonate, a carboxylate, and a phosphate.
18. The polymer complex of claim 1 , wherein A is selected from sulfopropyl, sulfobutyl, carboxypropyl, and carboxypentyl.
19. The polymer complex of claim 1 , wherein A is of the formula L-G, where L is a C2-C12 linear or branched alkyl linker and G is a negatively charged carboxy or sulfonate.
20. The polymer complex of claim 19 , wherein L is substituted with an aryl, alkoxy, alkenyl, alkynyl, —F, —Cl, —OH, aldehyde, ketone, ester, or amide.
21. The polymer complex of claim 1 , wherein D is styrene or C1-C18 alkyl methacrylate.
22. The polymer complex of claim 1 , wherein an average molecular weight of the second polymer is above 50,000.
23. The polymer complex of claim 1 , wherein an average molecular weight of the second polymer is above 200,000.
24. The polymer complex of claim 1 , wherein at least one constituent of the second polymer is independently selected from pyridine, imidazole, oxazole, thiazole, pyrazole, and any derivative thereof.
25. The polymer complex of claim 1 , where the second polymer is configured to limit a flux of glucose or lactate thereacross.
26. The polymer complex of claim 1 , wherein the second polymer is formed in situ on top of the first polymer.
27. An electrochemical sensor, comprising:
a working electrode comprising a polymer complex, the polymer complex comprising:
a first polymer disposed on top and in contact with the working electrode, the first polymer comprising an analyte responsive enzyme and a mediator, wherein the mediator is chemically bonded to the first polymer; and
a second polymer disposed on top and in contact with the first polymer, the second polymer comprising a polymer having the formula:
wherein the solid horizontal line represents a polymer backbone;
A is an alkyl substituted with a water-soluble constituent;
D is selected from phenylalkyl, alkoxystyrene, hydroxyalkyl, alkoxyalkyl, alkoxycarbonylalkyl, a poly(ethylene glycol)-containing constituent, and a polyhydroxyl-containing constituent; and
each of n, 1, p and q is independently a positive number; and
a counter electrode.