Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
1. An isolated GH61 polypeptide having cellulolytic enhancing activity, selected from the group consisting of:
(a) a GH61 polypeptide comprising an amino acid sequence having at least 95% sequence identity to amino acids 23 to 251 of SEQ ID NO: 2; and
(b) a GH61 polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 99% sequence identity to nucleotides 67 to 868 of SEQ ID NO: 1 or the cDNA sequence thereof.
2. The polypeptide of claim 1 , comprising amino acids 23 to 251 of SEQ ID NO: 2; or a fragment thereof having cellulolytic enhancing activity.
3. The polypeptide of claim 1 , which is encoded by the polynucleotide contained in plasmid pGEM-T-GH61 a51486 which is contained in E.coli DSM 22656 as deposited with the Deutsche Sammlunq von Mikroordanismen and Zellkulturen GmbH and given accession number DSM 22656.
4. An isolated recombinant host cell comprising an isolated polynucleotide comprising a nucleotide sequence that encodes the GH61 polypeptide of claim 1 .
5. A method of producing the GH61 polypeptide of claim 1 , said method comprising:
(a) cultivating an isolated Thermoascus crustaceus cell under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide.
6. A method of producing the GH61 polypeptide having cellulolytic enhancing activity of claim 1 , said method comprising: (a) cultivating a recombinant host cell comprising an isolated polynucleotide encoding the polypeptide under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide.
7. A method of producing the GH61 polypeptide of claim 1 , said method comprising: (a) cultivating a transgenic plant or a plant cell comprising an isolated polynucleotide encoding the polypeptide under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide.
8. A transgenic plant, plant part or plant cell transformed with a polynucleotide encoding the GH61 polypeptide of claim 1 , wherein the polypeptide comprises a signal peptide directing the polypeptide into the secretory pathway.
9. A method of producing a protein, said method comprising: (a) cultivating a recombinant host cell comprising a gene encoding the protein operably linked to an isolated polynucleotide encoding a signal peptide comprising or consisting of amino acids 1 to 22 or SEQ ID NO: 2, wherein the gene is foreign to the polynucleotide encoding the signal peptide, under conditions conducive for production of the protein; and (b) recovering the protein.
10. A method for degrading or converting a cellulosic material comprising treating the cellulosic material with an enzyme composition in the presence of the GH61 polypeptide having cellulolytic enhancing activity of claim 1 .
11. The method of claim 10 , further comprising recovering the degraded cellulosic material.
12. A method for producing a fermentation product, said method comprising:
(a) saccharifying a cellulosic material with an enzyme composition in the presence of the GH61 polypeptide having cellulolytic enhancing activity of claim 1 ;
(b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product; and
(c) recovering the fermentation product from the fermentation.
13. A method of fermenting a cellulosic material comprising fermenting the cellulosic material with one or more fermenting microorganisms, wherein the cellulosic material is saccharified with an enzyme composition in the presence of the GH61 polypeptide having cellulolytic enhancing activity of claim 1 .
14. The method of claim 13 , wherein the fermenting of the cellulosic material produces a fermentation product.
15. The method of claim 14 , further comprising recovering the fermentation product from the fermentation.
16. The polypeptide of claim 1 , comprising an amino acid sequence having at least 96% sequence identity to amino acids 23 to 251 of SEQ ID NO: 2.
17. The polypeptide of claim 16 , comprising an amino acid sequence having at least 97% sequence identity to amino acids 23 to 251 of SEQ ID NO: 2.
18. The polypeptide of claim 17 , comprising an amino acid sequence having at least 98% sequence identity to amino acids 23 to 251 of SEQ ID NO: 2.
19. The polypeptide of claim 18 , comprising an amino acid sequence having at least 99% sequence identity to amino acids 23 to 251 of SEQ ID NO: 2.
20. The polypeptide of claim 1 , consisting of amino acids 23 to 251 of SEQ ID NO: 2; or a fragment thereof having cellulolytic enhancing activity.
21. The polypeptide of claim 1 , which is encoded by a polynucleotide comprising a nucleotide sequence having at least 99% sequence identity to nucleotides 67 to 868 of SEQ ID NO: 1 or the cDNA sequence thereof.
22. A nucleic acid construct comprising an isolated polynucleotide comprising a nucleotide sequence that encodes the GH61 polypeptide of claim 1 operably linked to one or more heterologous control sequences that direct the production of the polypeptide in an expression host.
23. A recombinant expression vector comprising an isolated polynucleotide comprising a nucleotide sequence that encodes the GH61 polypeptide of claim 1 .