Composition and use of phyto-percolate for treatment of disease
This invention relates generally to a method of preparation of a phyto-percolate that is derived from fresh water mixture including algae. The phyto-percolate is believed to contain an enzyme having proteolytic activity. The invention further relates to the use of the phyto-percolate in a variety of disease state.
1. A method of producing a phytopercolate having enzymatic activity comprising the steps of: (a) producing a phytopercolate culture from microorganisms of ATCC Deposit #PTA-5863 and purified water; (b) feeding said phytopercolate culture with a nutrient; and (c) decanting said phytopercolate from said phytopercolate culture.
2. The method of claim 1 , wherein said phytopercolate culture comprises a mixture of approximately 100-200 mL of dense algal cells and approximately 10 Liters of purified water.
3. The method of claim 1 , wherein said enzymatic activity ranges from 15-50 mU/mL.
4. The method of claim 1 , wherein said phytopercolate is fed with nutrient sufficient liquid growth media.
5. The method of claim 1 , wherein said feeding comprises 1 mL per week of live active yeast liquid extract prepared from 1.0 g dry active yeast and 50 mL warm water.
6. The method of claim 1 , wherein said decanting occurs between the 5 th and 10 th day after said feeding.
7. The method of claim 1 , further comprising a step of filtering said phytopercolate.
8. The method of claim 7 , wherein said filtering comprises: a first stage of filtration through a filter having a 1 μm nominal pore size; a second stage of filtration through a filter having a 0.5 μm nominal pore size; and a final stage of filtration through sterile membrane filters having progressively smaller absolute pore sizes of 0.45 μm and 0.22 μm.
9. The method of claim 1 , wherein said nutrient comprises a liquid extract of live Saccharomyces cerevisiae.
10. A method of treating inflammation in a mammal, said method comprising the step of administering to said mammal a therapeutically effective amount of the phytopercolate prepared by the method of claim 1 wherein said inflammation is treated.
11. The method of claim 10 , wherein said mammal is a human, a dog, a cat, a cow, a pig, or a horse.
12. A method of treating obesity, diabetes, cancer, viral infections, oxidative stress, cardiovascular diseases, cerebral vascular diseases, immune system disorders, metabolic disorders, HIV, or gastric reflux disease in a mammal, said method comprising the step of administering to said mammal a therapeutically effective amount of the phytopercolate prepared by the method of claim 1 wherein said obesity, diabetes, cancer, viral infections, oxidative stress, cardiovascular diseases, cerebral vascular diseases, immune system disorders, metabolic disorders, HIV, or gastric reflux disease is treated.
13. The method of claim 12 , wherein said mammal is a human, a dog, a cat, a cow, a pig, or a horse.