Zinc finger nuclease for the CFTR gene and methods of use thereof
The present invention provides new zinc finger proteins and zinc finger nuclease (ZFNs) that find particular using in repairing the cystic fibrosis transmembrane conductance regulator (CFTR) gene.
1. A method of promoting recombination within a CTFR gene in a human cell comprising contacting said cell with a first zinc three-finger binding domain that targets a nucleotide sequence TTAGAAGTG (SEQ ID NO:132) and a second zinc three-finger binding domain that targets a nucleotide sequence TTATGGGAG (SEQ ID NO:133), wherein each of said first and second zinc three-finger binding domains are linked to a non-specific nuclease, wherein said first zinc three-finger binding domain comprises a sequence selected from SEQ ID NOS:3-5, 6-8, 9-11, 12-14, 15-17, 18-20, 21-23, 24-26, 27-29, 30-32, 33-35 and 36-38 for TTAGAAGTG (SEQ ID NO:132) and said second zinc three-finger binding domain comprises a sequence selected from the group consisting of SEQ ID NOS:39-41, 42-44, 45-47, 48-50, 51-53, 54-56, 57-59, 60-62, 63-65, 66-68, 69-71, 72-74, 75-77, 78-80, 81-83, 84-86, 87-89, 90-92, 91-93, 94-96, 97-99, 100-102, 103-105, 106-108, and 109-111 for TTATGGGAG (SEQ ID NO:133).
2. The method of claim 1 , wherein said human cell is a lung epithelial cell, and intestinal epithelial cell, a biliary duct epithelial cell, a gall bladder epithelial cell or pancreatic epithelial cell.
3. The method of claim 2 , wherein said epithelial lung cell or pancreatic cell comprises a CFTR gene with a ΔF508 mutation.
4. The method of claim 3 , wherein said epithelial lung cell or pancreatic epithelial cell is located in a living human subject.
5. The method of claim 4 , wherein contacting comprises administering said first and second zinc three-finger binding domains to lung or pancreatic tissue of said subject.
6. The method of claim 5 , wherein administration to lung tissue comprises inhalation or topical instillation.
7. The method of claim 5 , wherein administration to pancreatic tissue comprises injection.
8. The method of claim 1 , wherein contacting comprises administering to said subject an expression vector comprising a first nucleic acid segment encoding a first zinc three-finger binding domain that targets TTAGAAGTG (SEQ ID NO: 132) and a second nucleic acid segment encoding a second zinc three-finger binding domain that targets TTATGGGAG (SEQ ID NO: 133), said nucleic acids under the control of one or more promoters operable in a eukaryotic cell.
9. The method of claim 8 , wherein said vector is a viral vector.
10. The method of claim 9 , wherein said viral vector is an adenoviral vector, an adeno-associated viral vector, a pox viral vector, a herpes viral vector, a retroviral vector, a lentiviral vector.
11. The method of claim 10 , wherein the lentiviral vector is an integrase-deficient vector.
12. The method of claim of claim 8 , wherein each of said nucleic acid segments is under the control of a separate promoter active in said eukaryotic cell.
13. The method of claim 8 , wherein both of said nucleic acid segments are under the control of the same promoter.
14. The method of claim 12 , wherein said nucleic acid segments are separated by a transcription termination signal.
15. The method of claim 13 , wherein said nucleic acid segments are separated by an internal ribosome entry site and/or a picornavirus T2A sequence.