LIPOLYTIC ENZYME LIP1
The present invention provides a novel nucleic acid sequence, designated LIP1, encoding a lipolytic enzyme and the corresponding encoded amino acid sequences. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding at least one novel lipolytic enzyme, recombinant lipolytic enzyme proteins and methods for producing the same.
1 . A substantially pure polypeptide, wherein said polypeptide comprises an amino acid sequence having at least 75% amino acid sequence identity with an amino acid sequence set forth in SEQ ID NO.:3, wherein said polypeptide has lipolytic activity.
2 . The polypeptide of claim 1 , wherein said polypeptide comprises an amino acid sequence having at least 85% sequence identity with an amino acid sequence set forth in SEQ ID NO.:3.
3 . The polypeptide of claim 1 , wherein said polypeptide comprises an amino acid sequence having at least 95% sequence identity with an amino acid sequence set forth in SEQ ID NO.:3.
4 . The polypeptide of claim 1 , wherein said polypeptide comprises an amino acid sequence having at least 75% sequence identity with an amino acid sequence set forth in SEQ ID NO.:3 and is encoded by a polynucleotide having a nucleic acid sequence having at least 75% nucleic acid sequence identity with a nucleic acid sequence set forth in SEQ ID NO.:1.
5 . The polynucleotide of claim 4 , wherein said polynucleotide comprises a nucleic acid sequence having at least 85% nucleic acid sequence identity with a nucleic acid sequence set forth in SEQ ID NO.:1.
6 . The polynucleotide of claim 4 , wherein said polynucleotide comprises a nucleic acid sequence having at least 95% nucleic acid sequence identity with a nucleic amino acid sequence set forth in SEQ ID NO.:1.
7 . The polynucleotide of claim 4 , wherein said polynucleotide encoding a polypeptide is shown in FIG. 3 .
8 . A nucleic acid construct comprising the nucleic acid sequence of claim 4 , said nucleic acid sequence, which encodes for a polypeptide, being operably linked to one or more control sequences that direct the production of said polypeptide in a suitable host.
9 . A recombinant expression vector comprising the nucleic acid construct of claim 8 .
10 . A recombinant host cell comprising the nucleic acid construct of claim 8 .
11 . The host cell of claim 10 , wherein said host cell is E. coli.
12 . A detergent composition comprising the polypeptide of claim 1 .
13 . A method for producing a lipolytic enzyme comprising
a. transforming a host cell with a nucleic acid encoding a lipolytic enzyme;
said nucleic acid having at least 75% nucleic acid sequence identity with a nucleic acid sequence as set forth in SEQ ID NO.:1;
b. culturing the host cell under conditions to produce the lipolytic enzyme and
c. recovering the lipolytic enzyme.