Imaging electrophoresis system
A method employing gel electrophoresis and optical imaging techniques to measure the amount of biomaterial that attaches to specified locations on a detector slide such as a bioarray or biochip.
1. A method for identifying analyte molecules in a sample comprising:
providing an evanescent field imaging ellipsometry apparatus for creating an evanescent field above a surface of a bioarray having an array of probes which probes will be in the evanescent field and for spatial detection of binding events in the array with analyte molecules in a sample:
providing an electrophoresis apparatus for establishing an electrophoresis functioning electrical field extending in a direction laterally disposed to the bioarray and for placing a sample for moving analytes within the sample selectively by electrophoresis functioning laterally across the bioarray;
causing the sample to be exposed to the electrophoresis field whereby analyte molecules will transit within the electric field separated by the effect of the electric field;
selectively temporally detecting binding events of molecules that are moved by electrophoresis functioning across the bioarray.
2. The method of claim 1 further comprising:
causing transiting of analyte molecules by electrophoresis functioning in a first direction laterally with respect to the bioarray and later causing transiting of the analyte molecules by electrophoresis function in a second direction laterally with respect to the bioarray; whereby the first direction is selected to not cause transiting of analyte molecules over the bioarray and the second direction is selected to cause transiting of analyte molecules over the bioarray.
3. The method of claim 1 further comprising:
causing transiting of molecules by electrophoresis functioning initially in a gel and later leaving the gel into a selected fluid and then transiting over the bioarray.