Antibodies to IL-17A
View Patent ↗Engineered antibodies to human IL-17A are provided, as well as uses thereof.
1. An isolated nucleic acid encoding a binding compound that binds to human IL-17A, wherein the binding compound comprises (a) a light chain antibody variable region comprising the CDR sequences of SEQ ID NOs: 11, 12 and 13 and (b) a heavy chain antibody variable region comprising the CDR sequences of SEQ ID NOs: 14, 17 and 20.
2. The isolated nucleic acid of claim 1 , wherein the heavy chain variable region comprises the CDR sequences of SEQ ID NOs: 14, 16 and 19.
3. The nucleic acid of claim 2 , wherein the amino acid sequence of the light chain variable region is SEQ ID NO: 5 and the amino acid sequence of the heavy chain variable region is SEQ ID NO: 6.
4. The nucleic acid of claim 1 , comprising the sequences of SEQ ID NOs: 1 and 3, or the sequences of SEQ ID NOs: 62 and 63.
5. An expression vector comprising the nucleic acid of claim 3 operably linked to control sequences that are recognized by a host cell when the host cell is transfected with the vector.
6. The expression vector of claim 5 , wherein the binding compound comprises residues 1-220 of SEQ ID NO:2 and residues 1-454 of SEQ ID NO:4 expression vector has ATCC Accession No. PTA 7675.
7. An isolated A host cell comprising the vector of claim 6 .
8. A method of producing a polypeptide comprising:
culturing the host cell of claim 7 in culture medium under conditions wherein the nucleic acid sequence is expressed, thereby producing polypeptides comprising the light and heavy chain variable regions; and
recovering the polypeptides from the host cell or culture medium.