Thermostable catalase
An object is to efficiently produce thermostable catalase at low cost by expressing it as a recombinant protein in large quantity. A recombinant microorganism capable of efficiently expressing thermostable catalase can be provided by obtaining a DNA necessary for efficiently producing it as a recombinant protein, and the thermostable catalase can be efficiently produced at low cost by cultivating the obtained recombinant microorganism. Hydrogen peroxide can be efficiently decomposed at low cost, even at high temperature, by treating a solution containing hydrogen peroxide with the thermostable catalase of the present invention.
1. An isolated thermostable catalase produced by Penicillium pinophilum , selected from the group consisting of:
(i) a protein comprising the amino acid sequence consisting of amino acids 1-692 of SEQ ID NO: 2;
(ii) a protein comprising an amino acid sequence in which one to twenty amino acids are deleted, substituted, or added in the amino acid sequence consisting of amino acids 1-692 of SEQ ID NO: 2, and having a thermostable catalase activity;
(iii) a protein comprising an amino acid sequence having 90% or more identity with the amino acid sequence of a protein consisting of amino acids 1-692 of SEQ ID NO:2, and having a thermostable catalase activity;
(iv) a protein consisting of the amino acid sequence consisting of amino acids 1-692 of SEQ ID NO:2, and having a thermostable catalase activity; and
(v) the proteins (i) to (iv), having the amino acid sequence consisting of amino acids -1 to -42 of SEQ ID NO: 2, or an amino acid sequence in which one or two amino acids are deleted, substituted, or added in the amino acid sequence consisting of amino acids -1 to -42 of SEQ ID NO:2, at the N-terminal side of the protein.
2. The thermostable catalase according to claim 1 , having a molecular weight of approximately 80 kDa.
3. An isolated protein selected from the group consisting of:
(i) a protein comprising the amino acid sequence consisting of amino acids 1-692 of SEQ ID NO.: 2;
(ii) a protein comprising an amino acid sequence in which one to twenty amino acids are deleted, substituted, or added in the amino acid sequence consisting of amino acids 1-692 of SEQ ID NO.: 2, and having a thermostable catalase activity;
(iii) a protein comprising an amino acid sequence having 90% or more identity with the amino acid sequence of a protein consisting of amino acids 1-692 of SEQ ID NO.: 2, and having a thermostable catalase activity;
(iv) a protein consisting of the amino acid sequence consisting of amino acids 1-692 of SEQ ID NO.: 2, and having a thermostable catalase activity; and
(v) the proteins (i) to (iv), having the amino acid sequence consisting of amino acids -1 to -42 of SEQ ID NO.: 2, or an amino acid sequence in which one or two amino acids are deleted, substituted, or added in the amino acid sequence consisting of amino acids - 1 to - 42 of SEQ ID NO.: 2, at the N-terminal side of the protein.