Flow cytometry for high throughput screening
The present invention, provides a flow cytometry apparatus for the detection of particles from a plurality of samples comprising: means for moving a plurality of samples comprising particles from a plurality of respective source wells into a fluid flow stream; means for introducing a separation gas between each of the plurality of samples in the fluid flow stream; and means for selectively analyzing each of the plurality of samples for the particles. The present invention also provides a flow cytometry method employing such an apparatus.
1. A method for analyzing a plurality of samples, comprising:
moving a plurality of samples comprising particles into a fluid flow stream;
separating adjacent ones of said plurality of samples from each other in said fluid flow stream by a separation fluid, thereby forming a bubble-separated fluid flow stream;
guiding said bubble-separated fluid flow stream including said samples separated by said separation fluid to and through a flow cytometer; and
operating said flow cytometer to focus said bubble-separated fluid flow stream and to selectively analyze the particles in each of said plurality of samples as said bubble-separated fluid flow stream passes through said flow cytometer.