Materials and methods for hypothermic collection of whole blood
The present invention relates to materials and methods for hypothermic collection of whole blood, and components thereof, which can extend the holding time of blood beyond the current useable limit. Additionally, blood can be drawn directly into a hypothermic preservation solution without the addition of standard anticoagulants. This is enabled by providing sustained cellular viability under hypothermic conditions using a nutrient matrix devoid of animal proteins and containing energy substrates, free-radical scavengers, and impermeants that is ionically balanced for storage of biologic materials at low temperatures to prevent cellular stress-induced apoptosis.
1. A method of harvesting whole blood, the method comprising:
a) providing a volume of whole blood from a donor; and,
b) mixing, in a container, the volume of whole blood, or a portion thereof, with a volume of preservation solution adapted for cellular osmotic balance at hypothermic temperatures, such that the ratio of whole blood to preservation solution is at least about 1:0.1 by volume,
c) maintaining the mixture of whole blood and preservation solution at hypothermic temperatures,
wherein the volume of whole blood, the volume of preservation solution, and the container are free from an exogenously-introduced anticoagulant reagent, and
wherein the preservation solution comprises an ingredient selected from the group consisting of: an aqueous solution of electrolytes containing potassium ions at a concentration range of from about 35 to about 45 mM, sodium ions at a concentration range of from about 80 to about 120 mM, magnesium ions at a concentration range of from about 2 to about 10 mM, chloride ions at a concentration range of from about 15 to about 20 mM, and calcium ions at a concentration range of from about 0.01 to about 0.1 mM; an impermeant anion; mannitol; a macromolecular oncotic agent; at least one simple sugar; a substrate for the regeneration of ATP; a biological pH buffer effective under physiological hypothermic conditions; and combinations thereof.
2. The method of claim 1 , wherein the ratio of whole blood to preservation solution is at least about 1:0.5, 1:1, 1:2, 1:5, or 1:10.
3. The method of claim 1 , wherein the mixture of whole blood and preservation solution is maintained at about 2° C. to about 18° C.
4. The method of claim 1 wherein the preservation solution comprises an aqueous solution of electrolytes containing potassium ions at a concentration range of from about 35 to about 45 mM, sodium ions at a concentration range of from about 80 to about 120 mM, magnesium ions at a concentration range of from about 2 to about 10 mM, chloride ions at a concentration range of from about 15 to about 20 mM, and calcium ions at a concentration range of from about 0.01 to about 0.1 mM; an impermeant anion; mannitol; a macromolecular oncotic agent; at least one simple sugar; a substrate for the regeneration of ATP; and a biological pH buffer effective under physiological hypothermic conditions.
5. The method of claim 4 wherein the preservation solution further comprises glutathione, a vitamin E derivative, an antioxidant, and combinations thereof.
6. The method of claim 1 wherein the whole blood is cord blood.
7. The method of claim 1 , wherein the mixture of whole blood and preservation solution is maintained at about 2° C. to about 18° C. for at least 24 hours.
8. The method of claim 1 , wherein the mixture of whole blood and preservation solution is maintained at about 2° C. to about 18° C. for at least 48 hours.
9. The method of claim 1 , wherein the mixture of whole blood and preservation solution is maintained at about 2° C. to about 18° C. for at least 72 hours.
10. The method of claim 1 , wherein the mixture of whole blood and preservation solution is maintained at about 2° C. to about 18° C. for at least 7 days.
11. The method of claim 1 , wherein the mixture of whole blood and preservation solution is maintained at about 2° C. to about 18° C. for at least 14 days.
12. The method of claim 1 , wherein the mixture of whole blood and preservation solution is maintained at about 2° C. to about 18° C. for at least 21 days.